We propose a de novo glycan display approach that combines metabolic labeling and a glycancaging strategy as a facile editing method for cell-surface glycans. This method enables the introduction of antigen glycans onto cancer cells to induce immune responses through antibody recruiting. The caging strategy prevents the capture of α-rhamnose (an antigen glycan) by endogenous antibodies during the introduction of the glycan to the targeted cell surface, and subsequent uncaging successfully induces immune responses. Therefore, this study proposes a practical method for editing the cell-surface glycocalyx under promiscuous conditions, such as those in vivo, which paves the way for the development of glycan function analysis and regulation.
We propose a de novo glycan display approach that combines metabolic labeling and a glycan‐caging strategy as a facile editing method for cell‐surface glycans. This method enables the introduction of antigen glycans onto cancer cells to induce immune responses through antibody recruiting. The caging strategy prevents the capture of α‐rhamnose (an antigen glycan) by endogenous antibodies during the introduction of the glycan to the targeted cell surface, and subsequent uncaging successfully induces immune responses. Therefore, this study proposes a practical method for editing the cell‐surface glycocalyx under promiscuous conditions, such as those in vivo, which paves the way for the development of glycan function analysis and regulation.
Glycan Engineering. In their Communication (e202303750), Yoshiyuki Manabe, Koichi Fukase et al. report a glycan‐display approach that combines metabolic labeling and the glycan‐caging strategy for editing cell‐surface glycans. The method enables the introduction of antigen glycans onto cancer cells to induce immune responses.
Glycan Engineering. In their Communication (e202303750), Yoshiyuki Manabe, Koichi Fukase et al. report a glycan‐display approach that combines metabolic labeling and the glycan‐caging strategy for editing cell‐surface glycans. The method enables the introduction of antigen glycans onto cancer cells to induce immune responses.
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