Abstrak: Buah nanas (Ananas comosus (L) Merr) merupakan salah satu jenis buah-buahan tropis yang banyak diminati masyarakat. Buah nanas biasa dingunakan masyarakat sebagai antibakteri, antiinflamasi, antikoagulan, dan antikanker. Tujuan dari penelitian ini untuk mengetahui uji daya hambat anti bakteri air perasan daging buah nanas (Ananas comosus (L) Merr Var.  Queen) terhadap bakteri Propionibacterium acnes. Penelitian ini merupakan penelitian eksperimental laboratorium dengan menggunakan metode difusi kertas cakram. Hasil dari penelitian menunjukkan bahwa air perasan daging buah nanas (Ananas comosus (L) Merr Var. Queen) pada konsentrasi 25%, 50%, 75%, 100% memiliki efektivitas sedang sebagai antibakteri dalam menghambat pertumbuhan bakteri Propionibacterium acnes, pada kontrol positif yang menggunakan klindamisin dengan rata-rata diameter zona hambat sebesar 25,8 mm memiliki pengaruh antibakteri yang sangat kuat dan untuk kontrol negatif dengan menggunakan aquades tidak menunjukkan zona hambat 0 mm pada bakteri Propionibacterium acnes.Kata kunci: Buah nanas, propionibacterium acnes, daya hambat antibakteri.Abstract: Pineapple (Ananas comosus (L) Merr) is one of the most popular fruit among people, Pineapple is used as antibacterial, anti-inlfammatory, anticoagulant and anticancer. The purpose of this study is to determine the antibacterial inhibitory test of pineapple juice (Ananas comosus (L) Merr Var. Queen) against Propionibacterium acnes. This research is a laboratory experimental using paper disc diffisuion method. The results of this study indicate that pineapple juice at concentration of 25%, 50%, 75%, 100% it has a moderately antibacterial effectiveness in inhibit the growth of Propionibacterium acnes, positive control using clindamycin with an average inhibition zone diameter of 25.8 mm has a very strong antibacterial effect and for negative control using aquades does not show 0 mm inhibition zone in Propionibacterium acnes bacteria.Keywords: Pineapple fruit, propionibacterium acnes, antibacterial inhibition.

Pineapple fruit (Ananas comosus (L) Merr) is one type of tropical fruit that is consumed by many people. Pineapple is also commonly used by the community as an antibacterial, anti-inflammatory, anti-coagulant, and anti-cancer. The purpose of this study was to determine the effectiveness of the antibacterial inhibitory effect of pineapple juice (Ananas comosus (L) Merr Var. Queen) on Eschericia coli bacteria. This study used an experimental laboratory method with disc diffusion and using ciprofloxacin disc as a positive control and distilled water as a negative control. Based on the results of research that has been done, it can be concluded that pineapple juice (Ananas comosus (L) Merr Var. Queen) at a concentration of 25%, 50%, 75%, and 100% has the effectiveness as an antibacterial in inhibiting the growth of Eschericia coli bacteria.