Oleaginous fungi are one of the microorganisms that can accumulate a high number of biomasses quickly (within 96-130 hours) and are often used to produce lipids. The growth of fungi depends on the chemical composition of the environment in which it grows. The growth media of fungi must contain high carbohydrates as a source of nutrients and high nitrogen content. One of the carbon sources that fungi can use in the growth process is glucose. BR 2.2 isolate is an oleaginous fungus capable of accumulating 28.44% lipids from the total dry biomass with glucose as a carbon source in 50 mL of growth media. Therefore, this study was conducted to determine the effect of variations in the volume of media and incubation time on the production of biomass and lipid isolate BR 2.2. Biomass and lipid production were analyzed at media with additional water volumes of 10, 20, 30, 40, and 50 mL with 48, 96, and 144 hours of incubation times. The results showed that lipid accumulation and biomass production increased with the reduction of water content in the growth media and reached the highest number in the media volume of 20 mL with an incubation time of 144 hours, i.e., 0.87±0.04 g/L and 12.53±0.29 g/L. It can be concluded that biomass and fungal lipid increased along with incubation time and nutrient concentration.
Indonesia is a maritime country with high diversity of marine biota, which one is from Phylum Echinoderms. Echinoderms are known as biota of marine which is are living on corals, sandy, and on the intertidal zone and deep ocean. The intertidal characteristics of Sundak Beach has a suitable structure to echinodems life because there are many corals, but there is many species has not been identified before. The purpose of this research is to know the diversity of echinoderms phylum on the intertidal zone. Sampling was carried out on August, 24th 2019 with purposive random sampling method along the coastal-line in intertidal zone. The result of this research are identified and classified on the each class. Based on the results, the are found species from Echinodea, Holothuroidea, and Ophiuroidea. Species from Echinoidea are Tripneustes gratilla, Echinometra mathaei, Heterocentrotus trigonarius, Diadema antillarum., and Echinothrix calamaris. Holothuroidea namely Holothuria atra. Ophiuroidea namely Ophiotrix fragilis, Ophiocoma scolopendrina, and Ophiocoma erinaceus. The conclusion from this study is the discovery of five species from class Echinoidea, one species from class Holothuroidea and three species from class Ophiuroidea.
Echinoderm is one of the phylum which has high diversity and abundance in Indonesia, especially in Gunung Kidul, Yogyakarta. Sadranan and Slili beach are side by side beach located in Gunung Kidul. This beach is dominated by coral and rocky coral with macroalgae that provide the habitat of Echinoderms. This research is conducted to study the abundance and distribution pattern of Echinoderms in the intertidal zone between Sadranan and Slili beach. Sampling was carried out on Friday, 4 October 2019 at 05.00 p.m. in the intertidal zone using quadrat plot methods. The beach area is 2240 m2 which is divided into 21 plots with 1 x 1 m size. Data analysis was performed by measuring Margalef index (D), Pielou index (e), and Shannon-Wiener index (H’) then analyzing the distribution pattern and habitat parameters. The result shows that the Echinoderms which has the highest abundance is Echinometra mathei with 19 individual founds. The distribution pattern of Echinometra mathei, Stomopneustes variolaris, Heterocentrotus trigonarius, Ophiocoma erinaceus, Macrophiotrix longipeda, Ophiomastix annulosa, and Echinus sp. is clumped. The distribution pattern of Tripneustes gratilla, Diadema antillarum, Echinotrix calamaris, and Ophiocoma scolopendrina is uniform
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