The study done aimed to analyze the factors that could affect repurchase intention. The study aimed to measure the effect of service quality, brand image, and word of mouth on repurchase intention. Furthermore, the study was also to measure the most dominant factors in affecting repurchase intention, both direct and indirect effects. Semarang City was chosen as a representative place because it had the lowest room occupancy level among the provincial capitals in Java Island. The result of analysis shows that service quality, brand image, and word of mouth affected repurchase intention, both directly and indirectly. The most directly affecting variable was the word-of-mouth variable, while the variable that had indirect effect through the word-of-mouth variable was brand image. The contribution of all variables studied in affecting repurchase intention was 93.7 percent, it means that there were other factors affecting repurchase intention beyond the variables studied.
Botanic garden play a central role in the ex-situ plants conservation. It is important to maintain the survival life of the living plant collections. Knowledge on the details of reproductive biology of the living collections becomes vital to monitor the success or failure of conservation efforts. Tabebuia pallida (Lindl.) Miers. (Bignoniaceae) is one of the living plants collection in Purwodadi Botanic Garden that need to be monitored. So far information on the reproductive biology of this species is still limited. Based on that, in relation to the conservation effort, the objectives of this study were to provide knowledge about the reproductive biology of T. pallida. The research was conducted at Purwodadi BG, from September 2019 to February 2020. Several important aspects of reproductive biology, including flower biology, pollen viability and stigma receptivity, breeding system (Out-Crossing Index), and pollination biology were investigated. The results showed that T. pallida was monocious, has a pinkish tubular corolla with a yellow throat. There was no spatial separation between the anther and stigma. At the time of anthesis, both pollen and stigma have been receptive. Based on flower biology, pollen ornamentation (reticulate type), and the Out-Crossing Index (OCI=3), the flower showed some characters of entomophily, but self-pollination was possible. There are some insects visitor, but that acts as a pollinating agent was Vespula. The availability of effective pollinators in botanic garden was an obvious requirement for successful plants conservation.
The use of chemical insecticide in crop protection around the world has resulted in disturbances of the environment. Therefore, it is necessary to develop for environmentally friendly insect pest management techniques such as the activity of liquid smoke made from tobacco stem waste as an insecticide to Spodoptera litura. Activity of liquid smoke of tobacco stem waste was carried out at the laboratory condition. The results showed that the application of liquid smoke by using the spraying method (direct method) showed better results compared to the results of feeding method (indirect method). Lethal concentration (LC) at 5 days after treatment, LC 50 and LC 75 values the direct method of 2.9% and 8.87%, while the indirect method of 6,99% and 21.03%. The sub lethal concentration did not cause mortality of S. litura larvae, but inhibited the growth such as indicated by lower weight of larval and pupal in treated larvae than in control. Liquid smoke of tobacco stem waste has activity as an insecticide to S. litura.
<p>Entomopathogenic nematode of the families Steinernematidae and Heterorhabditidae is one of the best biological control agents of insect pests. Native isolates maybe more efficacious in controlling insect pests than imported ones because they have adapted to local environment. This study aimed to isolate and identify both nematode families from East Java and Bali<br />using DNA analysis. Sixty eight soil samples obtained from sandy soils in 16 sites of coastal regions and agricultural fields were tested for the presence of nematodes by baiting method with mealworm larvae (Tenebrio molitor). Five Heterorhabditis and two Steinernema were recovered from 7 soil samples (10% of total samples) of 7 sites (44% of total sites). Sequence analysis of<br />internal transcribed spacer (ITS) 1 and 2 regions of ribosomal DNA revealed that all Heterorhabditis belonged to indica species, with 99–100% nucleotide sequence similarities to published sequences. One of the Steinernema isolates had short infective juvenile body length (360 μm to 547 μm) and shared 99% nucleotide similarity to that of S. huense, a member of “carpocapsae” group. The other Steinernema isolate (DKS1) showed longer infective juvenile body length (from 548 μm to 762 μm) and shared 95% nucleotide similarity to the sequence of S. pakistanense, which belongs to “bicornutum” group. More detailed studies with respect to morphology are required for species confirmation of DKS1 isolate. Further exploration to<br />diverse habitat will likely to result in more previously unrecorded entomopathogenic nematode species in Indonesia.</p>
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