Microbial Fuel Cell (MFC) was a Technology that produced electrical energy utilizing microorganism. The purpose of this study was to know enceng gondok potency as substrate in MFC system and add electrolyte solution and buffer. This study used enceng gondok as substrate and Pseudomonas sp. bacteria. The Method of this study was using double compartment that consist of anode and cathode chambers. Both of them were related by salt bridge. The result showed that electric current and potential differences maximum that was produced (without added of electrolyte solution and buffer) was 0.11 mA and 0,27 V while power density was 20.34 mW/m2. Whereas Electrolyte solution and buffer combination showed electric current and potential differences maximum that produced was 3.06 mA and 0.86 V while power density yaitu 1802.46 mW/m2.
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