A new tumor-inhibitory antibiotic, 6-diazo-5-oxo-L-norleucine, has been isolated in crystalline form from culture broths of an unidentified Streptomyces. Characteristic physical properties of the compound have been determined. The structure of the antibiotic was proven by periodic acid oxidation to L-glutamic acid and Wolff rearrangement to -aminoadipic acid.
Butirosin, a new water-soluble aminoglycosidic antibiotic complex, active against gram-positive and gram-negative bacteria, has been isolated from fermentation filtrates of Bacillus circulans by ion exchange. The complex has been resolved into isomeric A and B components, C21H41N5012, by means of Dowex 1 X 1 or Dowex 1 X 2 chromatography. Properties ofthe free bases and the N, N', N", N"'-tetraacetyl derivatives of butirosin A and B are given.
Acid hydrolysis of vitamin B12 yields 5,6-dimethylbenzimidazole,1 5,6-dimethylbenzimidazole-1 --D-ribofuranoside,2 and its 2or 3-phosphate,3Dg-1 -amino-propanol-2,4 ammonia,6 and a red cobalt fraction.6 Comparable hydrolysis of crystalline pseudovitamin Bi27 does not give 5,6-dimethylbenzimidazole nor any derivative thereof. Hydrolysis with 1 M HC1 at 100°for 2.5-12 hours yields adenine, a purine tentatively identified as hypoxanthine, a ninhydrin reacting material cor-
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