The determination of the basic drug Amperozide, one of its main metabolites, and a third related compound in human blood plasma is described. All three compounds include an amine functionality. The process is governed by a moditled ASTED instrument (Gilson). The dialysis unit is exchanged for a supported liquid membrane (SLM), which gives a simultaneous cleanup and enrichment of the analytes. The ASI'ED is coupled to column liquid chromatographic equipment with W detection. An efficient cleanup is achieved, and there is no visible difference between the chromatograms after enrichment of a plasma blank and an aqueous blank. To increase the recovery, the same sample can be passed by the membrane several times, while the receiving phase is kept stagnant. An enrichment with three passages takes 15-20 min. The limit of detection for Amperozide in blood plasma is then -30 ng/mL. The use of the SLM technique for estimation of the degree of protein binding is discussed. Preliminary results agree reasonably with measurements with other techniques. The optimization of the enrichment with supported liquid membranes is described and different membrane supports are compared.When biological samples are analyzed, a cleanup procedure is usually necessary before any chromatographic determination. The most frequently used methods are liquid-liquid solvent extraction, solid phase extraction, SPE, or protein precipitation.' The former often gives a good cleanup from the matrix, but is either laborious or difficult to automate, and occasionally problems with emulsions can occur. The use of large amounts of organic solvents should also be avoided for environmental and health reasons. SPE is a convenient method?a but suitable packing materials are not always available, and the cleanup is sometimes not sufficiently efficient. With protein precipitation the sample is diluted, which increases the detection limit, and many endogenous compounds are still present in the sample after the removal of the proteins. A new pretreatment method is the use of restricted 5
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