The COVID-19 pandemic caused by SARS-CoV-2 has brought about an unprecedented crisis, taking a heavy toll on human health, lives as well as the global economy. There are no SARS-CoV-2-specific treatments or vaccines available due to the novelty of this virus. Hence, rapid development of effective vaccines against SARS-CoV-2 is urgently needed. Here we developed a pilot-scale production of a purified inactivated SARS-CoV-2 virus vaccine candidate (PiCoVacc), which induced SARS-CoV-2-specific neutralizing antibodies in mice, rats and non-human primates. These antibodies potently neutralized 10 representative SARS-CoV-2 strains, indicative of a possible broader neutralizing ability against SARS-CoV-2 strains circulating worldwide. Immunization with two different doses (3μg or 6 μ g per dose) provided partial or complete protection in macaques against SARS-CoV-2 challenge, respectively, without any antibody-dependent enhancement of infection. Systematic evaluation of PiCoVacc via monitoring clinical signs, hematological and biochemical index, and histophathological analysis in macaques suggests that it is safe. These data support the rapid clinical development of SARS-CoV-2 vaccines for humans. One Sentence Summary: A purified inactivated SARS-CoV-2 virus vaccine candidate (PiCoVacc) confers complete protection in non-human primates against SARS-CoV-2 strains circulating worldwide by eliciting potent humoral responses devoid of immunopathology 2017).

The high power generation cost impedes commercial-scale wave power operations. The main objective of this work was to provide a cost-sharing solution through combing the wave extraction and costal protection performance. A two-dimensional numerical wave tank was developed using Star-CCM+ Computational Fluid Dynamics software to investigate the hydrodynamic performance of a dual floater hybrid system consisting of a floating breakwater and an oscillating-buoy type wave energy converter (WEC). The new model was verified with published experimental results. The differences between the hydrodynamic performance of the hybrid system, a single WEC and a single breakwater were compared. The effects of the wave resonance in the WEC-breakwater gap and the geometrical parameters on the performance of the asymmetric WEC were also studied. It was found that the hybrid system demonstrated both better wave attenuation and wave energy extraction capabilities at low wave frequencies, i.e., wider effective frequency. The forces on the breakwater were generally reduced due to the WEC. Wave resonance in the narrow gap has an adverse effect on the hybrid system with an asymmetric WEC, while a beneficial effect with a symmetric WEC. The wave energy conversion efficiency of hybrid system can be improved by increasing the draft and width of the WEC and decreasing the distance between the WEC and the breakwater. The findings of this paper make wave energy economically competitive and commercial-scale wave power operations possible.

Combined floating breakwater and wave energy converter systems have the potential to provide a cost-effective solution to offshore power supply and coastal protection. This will make wave energy economically competitive and commercial-scale wave power operations possible. This paper investigates the hydrodynamic features of wave energy converters that meet the dual objectives of wave energy extraction and attenuation for such a combined system. A two-dimensional numerical model was established using Star-CCM+ commercial software based on viscous Computational Fluid Dynamics theory to investigate the hydrodynamic performance of an oscillating buoy Wave Energy Converter (WEC) type floating breakwater under regular waves. The model proposed in this paper was verified with published experimental results. The hydrodynamics of symmetric and asymmetric floaters were investigated to demonstrate their wave attenuation and energy extraction performance, including square bottomed, triangular bottomed (with and without a baffle plate), and the Berkley Wedge. The asymmetric floaters were found to have higher power conversion efficiency and better wave attenuation performance, especially the Berkeley Wedge bottom device and the triangular-baffle bottom device. The triangular-baffle bottom device with a simpler geometry achieved similar wave attenuation and energy extraction performance characteristics to that of the Berkeley Wedge device. The maximum energy conversion efficiency of the triangular-baffle bottom floater reached up to 93%, an impressive WEC device among many designs for wave energy conversion. There may be great potential for this newly proposed triangular-baffle bottom WEC type of floater to be an ideal coastal structure for both coastal protection and wave energy extraction.

Our objectives in this study were to perform separate proteomic analyses of porcine soft and hard enamel matrices, using the ProteomeLab PF-2D System, to compare the contents of the hard and soft enamel and to identify matrix constituents that are absent from the early maturation stage. Developing first permanent molars were dissected from 6-month-old pigs. Both immature and mature enamel samples were obtained by scraping the secretory-stage (soft) and maturation-stage (hard) enamel, respectively. Enamel matrix samples were sequentially extracted and fractionated with 50 mM phosphate buffer (pH 7.4) and then with 50 mM carbonate buffer (pH 10.8). The neutral enamel extract was separated into four fractions by successive ammonium sulfate precipitations. The alkaline enamel extract was separated into four fractions by ion-exchange chromatography. These eight extracts from both the soft and hard enamel were injected for chromatofocusing. Soft enamel fractions containing constituents absent from the hard enamel were further separated by reverse-phase high-performance liquid chromatography. The major soft enamel constituents absent from the hard enamel were acidic glycoproteins, corresponding to the 32-kDa enamelin, and the 29-, 27-, 15-, 13-, 8- and 6-kDa C-terminal fragments of ameloblastin. Loss of these glycoproteins is associated with a post-transition increase in enamel mineralization.

The current phase of the technological revolution and the accelerated rate of industrial change have encouraged the structural transformation of global infrastructure investment. This study aims to identify and evaluate the impact of new infrastructure investment on economic growth quality. This paper explains the theoretical mechanisms in terms of their effects on technological innovation, industrial structure, and productivity, using the three-dimensional analysis framework of economic growth conditions, process, and results. We then carry out an empirical examination based on provincial panel data for China from 2004 to 2019. The findings demonstrate that new infrastructure investment can significantly improve economic growth quality, and this conclusion still seems valid after conducting endogeneity treatments and robustness tests. Further mechanism evaluations indicate that new infrastructure investments contribute to boosting economic growth quality in terms of the condition, process, and results of economic growth by promoting technological innovation, improving industrial structure, and enhancing production efficiency. Moreover, the magnitude and mechanism of the positive effects differ depending on the heterogeneity of the region examined. In terms of the nonlinear change characteristics, the significant dual-threshold effect reveals that the marginal effects of new infrastructure investment on economic growth quality tend to be incremental under the threshold condition. This study provides a theoretical and factual basis for governments to enforce new infrastructure investments in the digital economy era, and it also has some value as a reference regarding the economic sustainability of developing countries.

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GRAPHICAL ABSTRACTThe composition and structural parameters of W/O microemulsions containing gemini surfactant 1,4-bis(3-dodecylimidazolium-1-yl)butane bromide [C 12 (Bim) 2 -2Br] and W/O microemulsions containing ionic liquid type surfactant 1-dodecyl-3-methylimidazolium bromide (C 12 mimBr) were studied and compared at different molar ratios of water to surfactant (x o ). The mole fractions of the alcohol in the interfacial layer X i a in C 12 (Bim) 2 -2Br-based systems are always larger than that in C 12 mimBr-based systems. However, the mole fractions of the alcohol in the oil phase X o a are nearly the same for both the systems. The C 12 (Bim) 2 -2Br-based systems have greater absolute values of the Gibbs energy (ÀDG o o!i ) than that of C 12 mimBr-based systems. In the C 12 (Bim) 2 -2Br-based systems, a large number of cosurfactants at the interfacial layer is conducive for the formation of a smaller droplet. The effects of alcohols, alkanes, and salinity on the composition and structural parameters of the two systems were also investigated and discussed.

Breast cancer (BC) is a leading cause of cancer-related mortality in females and is recognized as a molecularly heterogeneous disease. Previous studies have suggested that alternative messenger RNA (mRNA) processing, particularly alternative polyadenylation [poly(A)] (APA), can be a powerful molecular biomarker with prognostic potential. Therefore, in the present study, we profiled APA sites in the luminal B subtype of BC by sequencing APA sites (SAPAS) method, in order to assess the relation of these APA site-switching events to the recognized molecular subtypes of BC, and to discover novel candidate genes and pathways in BC. Through comprehensive analysis, the trend of APA site-switching events in the 3′ untranslated regions (3′UTRs) in the luminal B subtype of BC were found to be the same as that in MCF7 cell lines. Among the genes involved in the events, a significantly greater number of genes was found with shortened 3′UTRs in the samples, which were samples of primary cancer with relatively low proliferation. These findings may provide novel information for the clinical diagnosis and prognosis on a molecular level. Several potential biomarkers with significantly differential tandem 3′UTRs and expression were found and validated. The related biological progresses and pathways involved were partly confirmed by other studies. In conclusion, this study provides new insight into the diagnosis and prognosis of BC from the APA site profile aspect.