The major and minor allergens in fish and shellfish that have been identified are broadly spread in the myofibrillar and sarcoplasmic proteins. This study aimed to create a skin prick test reagent of crab myofibrillar and sarcoplasmic proteins to improve sensitivity and specificity diagnosis of allergy. Myofibrillar protein fraction was composed of 19 protein bands with the molecular weights of 9.6-111.6 kDa and sarcoplasmic protein fraction was composed of 19 protein bands with the molecular weights of 9.5-104.6 kDa. The allergenic myofibrillar protein has the molecular weights of 13.9-155.4 kDa while the allergenic sarcoplasmic protein has the molecular weights of 8.4-129.4 kDa. Crab myofibrillar and sarcoplasmic reagents met the requirements of the European Pharmacopoeia Monograph on Allergen Products 7 (2010:1063) for the parameters of moisture content, protein content, sterility and microbiology. A skin prick test to the respondents indicated that the sensitivity value of crab myofibrillar reagent was 85% and crab sarcoplasmic reagent was 69% with a negative error rate for each myofibrillar and sarcoplasmic reagents was 15% and 31%, respectively. The result diagnosis of crab myofibrillar and sarcoplasmic sensitivities would generate sensitivity value at 100%. The separation of crab proteins into myofibrils and sarcoplasm for skin prick test could improve its sensitivity value and lowered the negative misdiagnosis. The specificity of each myofibrillar and sarcoplasmic proteins was 100% with an error rate of positive diagnosis occurrence at 0%.
Companies in Indonesia have shareholders who are not dispersed or in other words the ownership is only held by one majority shareholder. This study examined the effects of investment decision on the firm value and the debt moderation on the effects of investment decisions on firm value. Debt moderation was used to test the agency conflict of debt use on investment decision. The company samples in this research were 90 companies. This research was conducted by using panel data regression with moderation. This study found that investment decision had a positive effect on firm value and the use of higher debt could lower the positive effect of investment decision on firm value.
ABSTRAKPerusahaan di Indonesia memiliki pemegang saham yang tidak tersebar atau dengan kata lain kepemilikan sahamnya hanya dipegang oleh satu pemegang saham mayoritas. Penelitian ini menguji pengaruh keputusan investasi terhadap nilai perusahaan dan moderasi utang pada pengaruh keputusan investasi terhadap nilai perusahaan. Moderasi utang digunakan untuk menguji konflik keagenan penggunaan utang pada pengambilan keputusan investasi. Sampel perusahaan pada penelitian ini sejumlah 90 perusahaan. Penelitian ini dilakukan dengan menggunakan regresi data panel dengan moderasi. Penelitian ini menemukan bahwa keputusan investasi berpengaruh positif terhadap nilai perusahaan dan penggunaan utang yang semakin tinggi menurunkan pengaruh positif keputusan investasi terhadap nilai perusahaan.
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