Cross-sections of Myristica malabarica (Lam) seed and mouse brain tissue were imprinted on such ordinary surfaces as printer paper and TLC plates, and successfully imaged by desorption electrospray ionization mass spectrometry (DESI-MS) at 250 mm resolution. Chemical images representing the distribution of the alkaloid malabaricone C in the seed substructures and individual lipids in the substructures of the brain were obtained. Practical implications include analysis of irregular or soft materials, easy recording, transportation and storage of the latent image, and posterior analysis of the samples by different techniques without the requirement of addition of matrices or use of specific types of surfaces.
Desorption electrospray ionization was employed for fast and direct ambient detection of the anti-tumor drug, camptothecin, and its derivative, 9-methoxycamptothecin in Nothapodytes nimmoniana. Different parts of the plant such as leaves, stems and bark were examined. The ion intensities suggest that the concentration in bark is higher than that in the leaves and stems. The method does not require any sample preparation or preseparation. The identity of the alkaloids was further confirmed by tandem mass spectrometry.
Basil (Ocimum sp.), a medicinal herb is used fresh and/or dry in various (culinary, cosmetic and pharmaceutical) preparations. Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. basilici is limiting basil cultivation in many countries. Since the leaf is the edible part in basil, new approaches are required to identify, and to prevent the spread of Fusarium pathogens. Desorption electrospray ionization mass spectrometry (DESI MS) was used for imaging thin layer chromatography (TLC)imprints of leaves of three different species of basil (Ocimum basilicum L., O. tenuiflorum L., and O. gratissimum L.), and the molecular manifestations during Fusarium contamination are recorded. DESI MS images showed the chemotaxonomic differences of basil species and the changes in metabolite ion peaks during pathogen infection. Besides easy detection of reported toxic metabolite(s) of the pathogen(s), the results include molecular images showing spatial distribution of all coexisting surfacebound metabolites in plant leaves, their fragment ions, and the transient changes in their spatial distribution during Fusarium attack under natural conditions. Demonstration of the same protocol to image seedling, young/mature leaves, basil/other related plant (Patchouli -Pogostemon cablin (Blanco)Benth.), wilt/other disease symptoms shows that prior knowledge of the metabolite profile of the plant/ pathogen is not required. This rapid detection method can be tailored to large scale screening programs for plant diseases suggesting potential implications in agriculture and quarantine requirements.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.