Background: Antiapoptotic Bcl-2 proteins are up-regulated in HTLV-1-infected T-cells. Results: Antiapoptotic Bfl-1 protein is regulated by viral Tax and HBZ proteins and is involved in survival of HTLV-1-transformed T-cells. Conclusion: Bfl-1, as well as Bcl-x L , induces resistance to apoptosis in HTLV-1-transformed T-cells. Significance: We provide evidence for Bfl-1 and Bcl-x L as potential therapeutic targets in the treatment of leukemia/lymphoma associated with HTLV-1.
PAGE 21360:Fig. 1B and supplemental Fig. S1B: The lysates from uninfected and HTLV-1-infected T-cell lines were loaded onto independent gels. After transfer, the membranes were cut according to the molecular weight markers to detect different proteins as shown on the figures. After revelation of actin, Bfl-1, Bcl-x L , and Tax for uninfected T-cells and Tax, Bcl-2, Bfl-1, and Bcl-x L for HTLV-1-infected T-cells, membranes were stripped and hybridized with actin and Bcl-2 for uninfected cells and actin for infected cells, respectively. The membranes for HTLV-1-infected cells were vertically spliced as two samples were removed as shown in the figure. PAGE 21365: Fig. 6C: We have removed the non-informative central part of the agarose gels. We only show the top and bottom of the gels corresponding to the supershift and the free DNA, respectively.
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