Background:In current times, enzyme-catalyzed reactions have gained importance for the development of new chemical processes. These require the production of large quantity of enzyme at low cost. Solid-state fermentation (SSF) is an efficient process because this bioprocess has a potential to convert agro-industrial residues into valuable compounds. Hence, the current study focuses on the optimization of process parameters for the higher production of laccase using a novel basidiomycete fungi Tricholoma giganteum AGHP under solid-state fermentation (SSF). Further, the purification of laccase using column chromatographic technique was performed.Results: Various physico-chemical parameters were evaluated and maximum production obtained was 2.69 × 10 5 U/g using wheat straw as a dry substrate. Optimum pH was found to be 5.0 and the temperature of 30 °C with 0.3 mM copper as an inducer. The enzyme was purified from the initial protein preparation by two-step column chromatography. A yield of 10.49 % with 3.33-fold purification was obtained using Sephadex G-75 gel permeation chromatography. Further increase in purification (total) was found to be 10.80-fold with a yield of 8.50 % using DEAE Sephadex A-50 ion exchange column chromatography. The purified enzyme was identified as a monomeric protein with a molecular weight of 66 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Conclusion:In view of the results obtained, we can conclude that the extracellular laccase production is governed by various cultural parameters such as pH, temperature, and the composition of culture medium. "One-factor-ata-time" methodology was capable of establishing the optimum conditions that significantly increases the enzyme production several folds using lignocellulosic substrate. Therefore, laccase from T. giganteum AGHP has a potential in several industrial applications.
The search for cost-effective and environmental benign practices for the production of bioactive compounds has gained considerable attention since last decade, due to increasing demand of eco-friendly processes. Many industries have started adopting routes for the development of green chemistry by employing enzymatic approaches to overcome the limitations of physico-chemical methods and environmental concerns. Laccase is one such enzyme which has gained considerable attention in recent years as a biocatalyst in organic synthesis. Laccases possess versatile biochemical properties and the reactions catalyzed by laccase require only molecular oxygen with concomitant release of water as a byproduct. They have been widely used for reactions such as dimerization, polymerization, coupling, and grafting reactions and for antibiotic modifications. This chapter summarizes the advances that have been made in developing technologies based on laccase mediated reactions in the field of medicine, agriculture, food, and pharmaceuticals.
The search for cost-effective and environmental benign practices for the production of bioactive compounds has gained considerable attention since last decade, due to increasing demand of eco-friendly processes. Many industries have started adopting routes for the development of green chemistry by employing enzymatic approaches to overcome the limitations of physico-chemical methods and environmental concerns. Laccase is one such enzyme which has gained considerable attention in recent years as a biocatalyst in organic synthesis. Laccases possess versatile biochemical properties and the reactions catalyzed by laccase require only molecular oxygen with concomitant release of water as a byproduct. They have been widely used for reactions such as dimerization, polymerization, coupling, and grafting reactions and for antibiotic modifications. This chapter summarizes the advances that have been made in developing technologies based on laccase mediated reactions in the field of medicine, agriculture, food, and pharmaceuticals.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.