Earlier studies have shown that from subcutaneous talc-induced granuloma in mice, a fraction could be extracted that fully protected mice against Lieia monocytogenes. Using standard biochemical procedures-i.e., ammonium sulfate fractionation, preparative electrophoresis, gel filtration chromatography, isoelectric focusing, and preparative polyacrylamide gel electrophoresis-we have now purified an active factor to homogeneity. A single band was obtained in NaDodSO4/poly--acrylamide gel with an apparent Mr of 55,000. It migrated with a1-globulins and the isoelectric point was 5 ± 0.1. The. biological activity was destroyed with Pronase but not with trypsin and a monospecific. polyclonal rabbit antiserum was obtained. The intravenous injection of 5 ,ug of this "mouse granuloma protein" fully protects mice against a lethal inoculum of L. monocytogenes.
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