Introduction:This research aimed to identify and quantify potentially pathogenic Vibrio from different cultivations of bivalve shellfi sh in the State of Santa Catarina, Brazil, and water regions in the South Bay, as well as correlate the incidence of these microorganisms with the physicochemical parameters of marine waters. Methods: Between October 2008 and March 2009, 60 oyster and seawater samples were collected from six regions of bivalve mollusk cultivation, and these samples were submitted for Vibrio counts. Results: Twenty-nine (48.3%) oyster samples were revealed to be contaminated with one or more Vibrio species. The Vibrio parahaemolyticus and Vibrio vulnifi cus counts in the samples ranged from < 0.5 log 10 Most Probable Number (MPN) g -1 to 2.3 log 10 MPN g -1 oyster and from < 0.5 log 10 MPN g -1 to 2.1 log 10 MPN g -1 oyster, respectively. Of the 60 seawater samples analyzed, 44 (73.3%) showed signs of contamination with one or more vibrio species. The counts of V. parahaemolyticus and V. vulnifi cus in the samples ranged from < 0.3 log 10 MPN·100mL -1 to 1.7 log 10 MPN·100mL -1 seawater and from < 0.3 log 10 MPN·100mL -1 to 2.0 log 10 MPN·100mL -1 seawater, respectively. A positive correlation between V. vulnifi cus counts and the seawater temperature as well as a negative correlation between the V. parahaemolyticus counts and salinity were observed.
Conclusions:The results suggest the need to implement strategies to prevent vibrio diseases from being transmitted by the consumption of contaminated bivalve shellfi sh.
The development of alternative microbiological techniques is driven by the necessity to meet the current needs to deliver rapid results in the manufacturing process of foods, but it is important that these methods be evaluated for each application. The objective of the present study was to assess the Petrifilm™ EB and the TEMPO® EB systems with ISO 21528-2:2004 for the count of Enterobacteriaceae in pasteurized and UHT milk samples. We analyzed the microflora of 141 pasteurized milk samples, 15 samples of artificially contaminated pasteurized milk and 15 samples of artificially contaminated UHT milk. Investigation of the method Petrifilm™ EB and ISO 21528:2 regression analysis showed a high correlation in the samples, r = 0.90 for the microflora of pasteurized milk, r = 0.98 for artificially contaminated pasteurized milk and r = 0.99 for the artificially contaminated UHT milk. In evaluating the system TEMPO EB ® method and ISO 21528:2 correlation was also significant in the analyzed samples, with r = 0.86 for the microflora of pasteurized milk, r = 0.96 for artificially contaminated pasteurized milk and r = 0.99 for artificially contaminated UHT milk. No statistically significant differences were observed between the three methods conducted to analyze artificially contaminated pasteurized and UHT milk at three inoculum levels. In conclusion, the Petrifilm™ EB system and the TEMPO® EB system may be an alternative to the ISO 21528-2:2004 for the Enterobacteriaceae assay for milk as because of the ease-of-operation and the time reduction achieved for conducting the microbiological assay using these systems.
This research aimed to identify and quantify Vibrio parahaemolyticus in fresh oysters (Crassostrea gigas), mussels (Perna perna) and seawater from different regions of cultivation of bivalve shellfishes in the seacoast of Santa Catarina, Brazil. Samples were collected between October 2012 and December 2013 and 130 oysters samples (Crassostrea gigas), 215 mussels samples (Perna perna) and 222 seawater were collected. The occurrence of V. parahaemolyticus in oysters and mussels was low, 10.76 and 11.62% of the samples tested. Higher incidences of V. parahaemolyticus were observed in seawater (18%). The density of V. parahaemolyticus in summer (December to March) was significantly greater than those in the other 3 seasons (P < 0.01). The occurrence of pathogenic V. parahaemolyticus in oyster, mussels and seawater was very low (<10%). It is recommend that control measures should be considered, including the establishment of an intensive and continuous monitoring of potentially pathogenic V. parahaemolyticus from all oyster-growing areas, the environmental parameters, and the assessment of the region-specific human health risk due to consumption of oyster.
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