Intracellular recordings were made from short segments of the muscular wall of the guinea-pig gastric antrum. Preparations were impaled using two independent microelectrodes, one positioned in the circular layer and the other either in the longitudinal layer, in the network of myenteric interstitial cells of Cajal (ICC MY ) or in the circular layer. Cells in each layer displayed characteristic patterns of rhythmical activity, with the largest signals being generated by ICC MY . Current pulses injected into the circular muscle layer produced electrotonic potentials in each cell layer, indicating that the layers are electrically interconnected. The amplitudes of these electrotonic potentials were largest in the circular layer and smallest in the longitudinal layer. An analysis of electrical coupling between the three layers suggests that although the cells in each layer are well coupled to neighbouring cells, the coupling between either muscle layer and the network of ICC MY is relatively poor. The electrical connections between ICC MY and the circular layer did not rectify. In parallel immunohistochemical studies, the distribution of the connexins Cx40, Cx43 and Cx45 within the antral wall was determined. Only Cx43 was detected; it was widely distributed on ICC MY and throughout the circular smooth muscle layer, being concentrated around ICC IM , but was less abundant in the circular muscle layer immediately adjacent to ICC MY . Although the electrophysiological studies indicate that smooth muscle cells in the longitudinal muscle layer are electrically coupled to each other, none of the connexins examined were detected in this layer.

We have found nifedipine-insensitive (NI), rapidly inactivating, voltage-dependent Ca2+ channels (current, NI-I(Ca)) with unique biophysical and pharmacological properties in the terminal branches of guinea pig mesenteric artery, by using a whole-cell mode of the patch-clamp technique. The fraction of NI-I(Ca) appeared to increase dramatically along the lower branches of mesenteric artery, amounting to almost 100% of global I(Ca) in its periphery. With 5 mmol/L Ba2+ as the charge carrier, NI-I(Ca) was activated with a threshold of -50 mV, peaked at -10 mV, and was half-activated and inactivated at -11 and -52 mV, respectively, generating a potential range of constant activation near the resting membrane potential. The NI-I(Ca) was rundown resistant, was not subject to Ca(2+)-dependent inactivation, and exhibited the pore properties typical for high voltage-activated Ca2+ channels; Ba2+ is approximately 2-fold more permeable than Ca2+, and Cd2+ is a better blocker than Ni2+ (IC(50), 6 and 68 micromol/L, respectively). Relatively specific blockers for N- and P/Q-type Ca2+ channels such as omega-conotoxins GVIA and MVIIC (each 1 micromol/L) and omega-agatoxin IVA (1 micromol/L) were ineffective at inhibiting NI-I(Ca), whereas nimodipine partially (10 micromol/L; approximately 40%) and amiloride potently ( approximately 75% with 1 mmol/L; IC(50); 107 micromol/L) blocked the current. Although these properties are reminiscent of R-type Ca2+ channels, expression of the alpha(1E) mRNA was not detected using reverse transcriptase-polymerase chain reaction. These results strongly suggest the predominant presence of NI, high voltage-activated Ca2+ channels with novel properties, which may be abundantly expressed in peripheral small arterioles and contribute to their tone regulation.

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SUMMARY1. Brief transmural stimuli, 05-1 ms, initiated contractions of the longitudinal muscle taken from the guinea-pig ileum that were recorded isometrically. In separate preparations similar stimuli were found to initiate excitatory junction potentials which were recorded using intracellular recording electrodes. All of these responses were abolished by either tetrodotoxin, wo-conotoxin or hyoscine.2. The contractions produced by increasing [K+]. were blocked by nifedipine,