3‐Chlorobiphenyl‐degrading bacteria were obtained from the mating between Pseudomonas putida strain BN10 and Pseudomonas sp. strain B13. Strains such as BN210 resulted from the transfer of the genes coding the enzyme sequence for the degradation of chlorocatechols from B13 into BN10, whereas B13 derivatives such as B131 have acquired the biphenyl degradation sequence from BN10. During growth of the hybrid strains on 3‐chlorobiphenyl 90% chloride was released. Activities of phenylcatechol 2,3‐dioxygenase, benzoate dioxygenase, catechol 1,2‐dioxygenase, chloromuconate cyloisomerase and 4‐carboxymethyl‐enebut‐2‐en‐4‐olide hydrolase were found in 3‐chlorobiphenyl‐grown cells. The hybrid strains were found to convert some congeners of the Aroclor 1221 mixture such as mono‐ and dichloro‐substituted biphenyls.
(1997). Aerobic degradation of polychlo-rinated biphenyls by Alcaligenes sp. JB1: metabolites and enzymes. Biodegradation, (7), 435-443. General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Key words: aerobic, biodegradation, enzymes, induction, polychlorinated biphenyls, resting-cell assay AbstractIn contrast to the degradation of penta-and hexachlorobiphenyls in chemostat cultures, the metabolism of PCBs by Alcaligenes sp. JB1 was shown to be restricted to PCBs with up to four chlorine substituents in resting-cell assays. Among these, the PCB congeners containing ortho chlorine substituents on both phenyl rings were found to be least degraded. Monochloro-benzoates and dichlorobenzoates were detected as metabolites. Resting cell assays with chlorobenzoates showed that JB1 could metabolize all three monochlorobenzoates and dichlorobenzoates containing only meta and para chlorine substituents, but not dichlorobenzoates possessing an ortho chlorine substituent. In enzyme activity assays, meta cleaving 2,3-dihydroxybiphenyl 1,2-dioxygenase and catechol 2,3-dioxygenase activities were constitutive, whereas benzoate dioxygenase and ortho cleaving catechol 1,2-dioxygenase activities were induced by their substrates. No activity was found for pyrocatechase II, the enzyme that is specific for chlorocatechots. The data suggest that complete mineralization of PCBs with three or more chlorine substituents byAlcaligenes sp. JB1 is unlikely.Abbreviations: PCB -polychlorinated biphenyls, CBA -chlorobenzoate, D -di-, Tr -tri-, Te -tetra-, Pe -penta-, H -hexa
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