Mouse models contribute in our understanding of human diseases. By this way, several strains of systemic lupus erythematosus (SLE)-prone mice including New Zealand Black (NZB), F1 hybrids of NZB x New Zealand White (NZW) (B/W F1), MRL/Mp-lpr/lpr (MRL/lpr), and BXSB mice are important in the research of the autoimmune diseases. Several genes and signaling pathways molecules have been related with SLE pathologies such as: Lyn, CD22, SHP-1 or the sle1, sle2 and sle3 congenic models. CD38 is a transmembrane receptor able to induce activation, proliferation, and survival of human and mouse lymphocytes; this molecule is expressed along the B cell ontogeny, in addition, CD38 is an ectoenzyme involved cell adhesion and is used as a disease marker for leukemia and myeloma, also is a dependable negative prognostic marker for chronic lymphocytic leukemia (CLL). The function of CD38 in autoimmunity has been proposed in human but there are not reports that clear the authentic role of this molecule in SLE. To study the implication of CD38 in the SLE pathogenesis we compare the proteinuria, serum ANA and anti-dsDNA antibody production, body-weight, nephritis and mortality in the CD38-deficient compared to the wild type (WT) mice. Subsequently, we expect that the pathologic manifestations of nephritis appear significantly earlier in the WT mouse as well survival rate changes.
CD38 is a 45 KD transmembrane protein, expressed by immature and mature lymphocytes. Agonistic antibodies directed to this molecule induce several biological responses: cellular proliferation, protein phosphorylation, intracellular Ca2+ mobilization, rescue from and induction of apoptosis. CD38 ligation with a mAb anti-CD38 (NIM-R5) induces apoptosis of the murine pro-B cell line (Ba/F3); this phenomenon depends on tyrosine kinases activation and the MAP kinase ERK. The expression and function of CD38 during B-cell ontogeny has been poorly analyzed in mice. We analyzed the expression of CD38 during the ontogeny of B cells in murine bone marrow. CD38 is expressed from the preproB stage. ProB, preB and immature B cells from murine bone marrow were all positive for the expression of CD38, but immature B cells had the highest fluorescence intensity, and the proB stage shows a low and high expression of CD38 that suggest two probable populations. The results suggest that CD38 may play a role during the ontogeny of murine B cells. We are analysing whether CD38 ligation on these subpopulations, induces proliferation, maturation or apoptosis.
CD38 is a 45 KD transmembrane protein, expressed by immature and mature lymphocytes. Agonistic antibodies directed to this molecule induce several biological responses: cellular proliferation, protein phosphorylation, intracellular Ca2+ mobilization, rescue from and induction of apoptosis. CD38 ligation with a mAb anti-CD38 (NIM-R5) induces apoptosis of the murine pro-B cell line (Ba/F3); this phenomenon depends on tyrosine kinases activation and the MAP kinase ERK. The expression and function of CD38 during B-cell ontogeny has been poorly analyzed in mice. We analyzed the expression of CD38 during the ontogeny of B cells in murine bone marrow. Bone marrow cells from C57BL/6J mice were stained with antibodies against: CD19, CD45R, IgM, CD43 and CD38. The samples were captured in a Beckman coulter’s CyAn flow cytometer and each subpopulation was analyzed with FlowJo v.7.5 software. CD38 is expressed from the preproB stage. ProB, preB and immature B cells from murine bone marrow were all positive for the expression of CD38, but immature B cells had the highest fluorescence intensity. The results suggest that CD38 may play a role during the ontogeny of murine B cells. Then, the next step is to analyze whether CD38 ligation on these subpopulations, induces proliferation, maturation or apoptosis.
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