: 07-11-2014: 07-11- Aceptación: 11-07-2015 Abstract Introduction: feeding for sheep production in the tropics accounts from 48 to 90% of total production cost; additionally, the use of grains also makes producers dependent on the international market. Moderate weight gains in rams could be obtained with foliage from tropical trees; however, some plants such as Hibiscus Rosa-sinensis have compounds that could act as phytoestrogens, which could compromise its potential use as animal feed.Method: twelve Pelibuey rams, aged 24±3 months old, 45±6 kg live weight and a body condition score of 2.3±0.3 in a scale from 1 to 5, were assigned to one of two treatments for 45 days. T1 (n = 12), commercial feed (500 g d -1 by sheep) with 16% CP, and T2 (n = 12), 20% of H.rosasinensis (451 g DM kg MV ~ 1.6 kg d-1 per animal). Both treatments were supplemented with (Pennisetum purpureum x P. Typhoides var. grass. Parameters of semen quality were evaluated: ejaculate volume (Vol, mL) and concentration (EC, millions per mL) and eight individual motility parameters were used: curvilinear speed (VCL, µ/s), straight line velocity Calidad seminal en ovinos pelibuey con inclusión de Hibiscus rosa-sinensis en la dieta
ABSTRACT. The seed of chia plant (Salvia hispanica) is characterized by a high content of polyunsaturated fatty acids, which is an important source of α-linolenic acid for human and animal consumption. During the harvest of the seed, waste is generated that contains remnants of leaves, stems and some seeds, which is generally discarded. The objective of the present work was to evaluate the eect of chia seed residue (CSR) on protein expression of the rabbit immune system, when added as a supplement in the diets of animals deliberately induced to an intestinal infection. Twenty rabbits (New Zealand × California) were randomly distributed in ve treatments and fed ve diets with dierent percentages of CSR inclusion (0, 10, 20, 30 and 40%). Quantication of the relative expression of the anti-inammatory Interleukin-10 (IL-10) and Tumor Necrosis Factor (TNF-α) genes showed that as CSR inclusion levels increased up to 40%, mRNA expression of IL-10 increased signicantly (22.4-fold) with respect to the control, while TNF-α mRNA expression was inversely proportional, where a signicant increase (7.47-fold) in mRNA expression was found in the control group. It is concluded that the indirect inclusion of α-linolenic acid through the consumption of CSR induces a positive response in the immune system of the rabbits and represents an alternative for the formulation of rations with nutraceutical eects.Key words: Feeding, α-linolenic acid, IL-10, TNF-α, Relative expression.RESUMEN. La semilla de la planta de la chía (Salvia hispánica), se caracteriza por su alto contenido de ácidos grasos poliinsaturados, por lo que es una fuente importante de ácido α-linolénico para consumo humano y animal. Durante la cosecha de la semilla, generan residuos que contiene remanentes de hojas, tallos y semillas, que son desechadas. El objetivo del presente trabajo fue evaluar el efecto del residuo de la cosecha de semilla de chía (RCS) sobre la expresion de proteínas del sistema inmune de conejos, al añadirlo como complemento en las dietas de animales inducidos con infección intestinal. Se distribuyeron al azar veinte conejos (Nueva Zelanda × California) en cinco tratamientos y se alimentaron con cinco dietas con porcentajes de inclusión de RCS de 0, 10, 20, 30 y 40%. La cuanticación de la expresión relativa de los genes interleuquina-10 (IL-10) y factor de necrosis tumoral (TNF-α) mostraron que conforme aumentaron los niveles de inclusión RCS hasta 40%, también aumentó de forma signicativa la expresión relativa de IL-10 hasta 22.4 veces con respecto al control, mientras que la expresión relativa de la TNF-α fue inversamente proporcional, presentando la mayor expresión relativa el grupo control con 7.47, por lo que la inclusión indirecta de ácido α-linolénico a través del consumo de RCS inducen una respuesta positiva en el sistema inmune de los conejos y representa una alternativa para la formulación de raciones con efectos nutracéuticos.
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