Abstract The used of chemical insecticides for mosquito control has caused resistance in themosquito populations. The aims of this study are to find out Lethal Concentration of non-polarextract from pomelo (Citrus maxima) leaf against mosquito larvae after 24 hoursexposure. Aedes aegypti and Culex quinquefasciatus larvae were tested in the study A.Larvicidal assay was using 20 larvae for each concentration in 100 ml solution with 5replications. The non-polar extract was tested at concentrations of 0 ppm, 500 ppm, 1375ppm, 2250 ppm, 3125 ppm, and 4000 ppm. The number of larvae mortality wasdetermined after 24 hours exposure. The dead larvae were counted and the data wasanalyzed using probit analyze. The results showed that non-polar extract from Citrusmaxima Leaf has potential larvicidal, LC90 = 880 ppm for mortality of Aedes aegypti larvae, LC90 = 408 ppm for mortality of Culex quinquefasciatus larvae, the non-polar extract is more toxic against Culex quinquefasciatus than Aedes aegypti larvae. The non-polar extract from pomelo (Citrus maxima) leaf has the potential of being developed aslarvicides for mosquito control. Abstrak Penggunaan insektisida kimia untuk mengontrol nyamuk dapat menyebabkanresistensi pada populasi nyamuk. Tujuan dari penelitian ini adalah untuk menemukannilai Konsentrasi Letal (LC90 ) ekstrak non polar daun jeruk bali (Citrus maxima) terhadap nyamuk setelah 24 jam. Larva yang digunakan dalam penelitian ini adalahlarva Aedes aegypti dan Culex quinquefasciatus. Kematian larva dihitung setelah 24 dan 48 jam.Uji larvasida menggunakan 20 ekor larva untuk setiap konsentrasi di dalam 100ml larutan ekstrak dengan lima replikasi. Ekstrak non polar diuji dengan konsentrasi 0ppm, 500 ppm, 1.375 ppm, 2.250 ppm, 3.125 ppm, dan 4.000 ppm. Angka kematianlarva dihitung setelah 24 jam paparan. Data dihitung dan dianalisa dengan analisisprobit. Hasil penelitian menunjukkan bahwa ekstrak non polar daun Citrus maximaberpotensi sebagai larvasida, Nilai LC90 = 880 ppm untuk kematian larva nyamuk Aedesaegypti, Nilai LC90 = 408 ppm untuk kematian larva nyamuk Culex quinquefasciatus, Ekstrak non polar lebih toksik terhadap larva Culex quinquefasciatus daripada larva Aedes aegypti. Ekstrak non polar dari daun Citrus maxima memiliki potensi untukdikembangkan sebagai larvasida untuk pengendalian nyamuk.
The vector-borne diseases transmitted by the mosquito of Aedes aegypti are considered causes of serious health problems. Moreover, the used of chemical insecticides for Ae. aegypti control has resulted in resistance in mosquito populations and environmental problem. The aims of this study are to evaluate the toxicity of methanol extract (CH3OH) from key lime leaves against Ae. aegypti larvae after 24 hours exposure. This was experimental laboratory study, with six treatments and five replicates. Larvae mortality was observed after 24 hours exposure. Then, data were analyzed by descriptive analyze and probit. visualization of the damage that occurs in the body was observed through microscope. The result showed that mortality of larvae increased with an increase of the concentration of extract. LC50 and LC99 of the extract are 2,197 ppm and 4,266 ppm. The extract can cause morphological damages of larvae, such as head lost, neck elongation, exo-skeleton lost, abdomen transparent, digestive tract full of extract, and loss of setae on the side of the body. In conclusion, citrus aurantifolia leaves methanol extract cause mortality and morphological damages of larvae
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