Sub-Saharan Africa is experiencing rapid urbanisation and many urban residents use groundwater where piped supplies are intermittent or unavailable. This study aimed to investigate long-term changes in groundwater contamination hazards and hand-dug well water quality in two informal settlements in Kisumu city, Kenya. Buildings, pit latrines, and wells were mapped in 1999 and 2013–2014. Sanitary risk inspection and water quality testing were conducted at 51 hand-dug wells in 2002 to 2004 and 2014. Pit latrine density increased between 1999 and 2014, whilst sanitary risk scores for wells increased between 2002 to 2004 and 2014 (n = 37, Z = −1.98, p = 0.048). Nitrate levels dropped from 2004 to 2014 (n = 14, Z = −3.296, p = 0.001), but multivariate analysis suggested high rainfall in 2004 could account for this. Thermotolerant coliform counts dropped between 2004 and 2014, with this reduction significant in one settlement. Hand-dug wells had thus remained an important source of domestic water between 1999 and 2014, but contamination risks increased over this period. Water quality trends were complex, but nitrate levels were related to both sanitary risks and rainfall. Given widespread groundwater use by the urban poor in sub-Saharan Africa, the study protocol could be further refined to monitor contamination in hand-dug wells in similar settings.

Highlights 1 We compared number size distributions from ELPI, SMPS, FMPS and APS 2  Results from four lab generated aerosols were compared in a wind tunnel 3  Good correlation was found between instruments in their middle size ranges 4  At the lower and upper particle diameters there were divergences 5  Particle type (size and shape) affected the correlation between instruments 6 7 8 4 Abstract 1

In urban slumshome to approximately 1 billion people worldwide-access to clean drinking water is woefully inadequate despite the United Nations' declaration that access to safe water is a fundamental human right. Households in slums are frequently forced to rely on multiple drinking water sources to meet their needs. Numerous factors influence choice of water source, including water quality, availability, reliability, and affordability. These factors are not temporally static, but rather will vary over multiple timescales (from sub-daily changes to annual changes and beyond) in response to changes in the water source itself and changes in the household's ability to use that source. For example, the cost of water can change over time in response to water availability (e.g. rainy season versus dry season) and a slum household's ability to pay for water will often change over time in response to changes in household income. However, existing national and global monitoring of safe water access, including Sustainable Development Goal 6, overlook these temporal dynamics of water access, quality and health risk in slums. This paper proposes a research agenda for exploring temporal changes in drinking water access and quality in urban slums and their potential influence on health risk. It argues that in the design of research studies, policy interventions, and drinking water monitoring aimed at improving access and health in urban slums, temporal dynamics 2 should be considered over at least three interlinked time scales: short-term (from sub-daily to week-to-week), medium-term (from month-to-month to season-toseason) and long-term (from year-to-year). The paper concludes with recommendations for future research on temporal dynamics of drinking water and health in slums.

Shallow hand-dug wells are commonly used to supplement partial or intermittent piped water coverage in many urban informal settlements in sub-Saharan Africa. Such wells are often microbially contaminated. This study aimed to quantify the amount of such groundwater consumed, identify the socio-economic profile of well owners and consumers, and patterns of domestic water usage in informal settlements in Kisumu, Kenya. Building on a previous study, 51 well owners and 137 well customers were interviewed about well water abstraction, water usage and handling patterns, asset ownership, and service access. An estimated 472m3 of groundwater per day was abstracted in two informal settlements, with most groundwater consumers using this water for purposes other than drinking or cooking. According to an asset index, well owners were significantly wealthier than both the customers purchasing their groundwater and those drinking or cooking with untreated groundwater. This suggests that shallow groundwater sources provide poorer urban households with a substantial volume of water for domestic purposes other than drinking and cooking. Ongoing challenges are thus to raise awareness of the health risks of such water among the minority of consumers who consume untreated groundwater and find means of working with well owners to manage well water quality

Innate behavioral biases such as human handedness are a ubiquitous form of inter-individual variation that are not strictly hardwired into the genome and are influenced by diverse internal and external cues. Yet, genetic and environmental factors modulating behavioral variation remain poorly understood, especially in vertebrates. To identify genetic and environmental factors that influence behavioral variation, we take advantage of larval zebrafish light-search behavior. During light-search, individuals preferentially turn in leftward or rightward loops, in which directional bias is sustained and non-heritable. Our previous work has shown that bias is maintained by a habenula-rostral PT circuit and genes associated with Notch signaling. Here we use a medium-throughput recording strategy and unbiased analysis to show that significant individual to individual variation exists in wildtype larval zebrafish turning preference. We classify stable left, right, and unbiased turning types, with most individuals exhibiting a directional preference. We show unbiased behavior is not due to a loss of photo-responsiveness but reduced persistence in same-direction turning. Raising larvae at elevated temperature selectively reduces the leftward turning type and impacts rostral PT neurons, specifically. Exposure to conspecifics, variable salinity, environmental enrichment, and physical disturbance does not significantly impact inter-individual turning bias. Pharmacological manipulation of Notch signaling disrupts habenula development and turn bias individuality in a dose dependent manner, establishing a direct role of Notch signaling. Last, a mutant allele of a known Notch pathway affecter gene, gsx2, disrupts turn bias individuality, implicating that brain regions independent of the previously established habenula-rostral PT likely contribute to inter-individual variation. These results establish that larval zebrafish is a powerful vertebrate model for inter-individual variation with established neural targets showing sensitivity to specific environmental and gene signaling disruptions. Our results provide new insight into how variation is generated in the vertebrate nervous system.

Innate behavioral biases such as human handedness are a ubiquitous form of inter-individual variation that are not strictly hardwired into the genome and are influenced by diverse internal and external cues. Yet, genetic and environmental factors modulating behavioral variation remain poorly understood, especially in vertebrates. To identify genetic and environmental factors that influence behavioral variation, we take advantage of larval zebrafish light-search behavior. During light-search, individuals preferentially turn in leftward or rightward loops, in which directional bias is sustained and non-heritable, and maintained by a habenula-rostral PT circuit. Here we use a medium-throughput recording strategy and unbiased analysis to show that significant individual to individual variation exists in wildtype larval zebrafish turning preference. We classify stable left, right, and unbiased turning types, with most individuals exhibiting a directional preference. Raising larvae at elevated temperature selectively reduces the leftward turning type and impacts rostral PT neurons, specifically. Exposure to conspecifics, variable salinity, environmental enrichment, and physical disturbance does not significantly impact inter-individual turning bias. Pharmacological manipulation of Notch signaling and carrying a mutant allele of a known Notch pathway affecter gene, gsx2, disrupted turn bias individuality in a dose-dependent manner. These results establish that larval zebrafish is a powerful vertebrate model for inter-individual variation with sensitivity to specific environmental perturbations and gene dosage.

This study assessed the level of heavy metal in roadside dust and PM mass concentrations along Thika superhighway in Kenya. Thika superhighway is one of the busiest roads in Kenya, linking Thika town with Nairobi. Triplicate road dust samples collected from 12 locations were analysed for lead (Pb), chromium (Cr), cadmium (Cd), nickel (Ni), zinc (Zn), and copper (Cu) using atomic absorption spectrophotometry (AAS). PM samples were collected on pre-weighed Teflon filters using a BGI personal sampler and the filters were then reweighed. The ranges of metal concentrations were 39-101 μg/g for Cu, 95-262 μg/g for Zn, 9-28 μg/g for Cd, 14-24 μg/g for Ni, 13-30 μg/g for Cr, and 20-80 μg/g for Pb. The concentrations of heavy metals were generally highly correlated, indicating a common anthropogenic source of the pollutants. The results showed that the majority of the measured heavy metals were above the background concentration, and in particular, Cd, Pb, and Zn levels indicated moderate to high contamination. Though not directly comparable due to different sampling timeframes (8 h in this study and 24 h for guideline values), PM for all sites exceeds the daily WHO PM guidelines of 25 μg/m. This poses a health risk to people using and working close to Thika superhighway, for example, local residents, traffic police, street vendors, and people operating small businesses. PM levels were higher for sites closer to Nairobi which could be attributed to increased vehicular traffic towards Nairobi from Thika. This study provides some evidence of the air pollution problem arising from vehicular traffic in developing parts of the world and gives an indication of the potential health impacts. It also highlights the need for source apportionment studies to determine contributions of anthropogenic emissions to air pollution, as well as long-term sampling studies that can be used to fully understand spatiotemporal patterns in air pollution within developing regions.

Electrical mini-grids can provide electrification to rural communities far from the national network. However the benefits of such schemes are disputed. We observed changes in two matched trading-centres in Makueni County, Kenya, neither of which were initially electrified. During the study a solar photovoltaic mini-grid scheme (13.5 kWp) was constructed in one of the trading-centres. After electrification there were relative increases in the number of businesses and business income. Comparing the households in the areas around the trading centres, perceived wealth increased more around the electrified trading centre. Qualitative interviews indicated improvements in service provision by the local school and health centre. The co-operative set up to run the mini-grid was free to set its own kWh tariff and chose to reduce it to a level that covers operating costs and would recover 70% of the initial investment interest-free. However, the tariff finally agreed is higher than the national grid tariff, which would be difficult to achieve if the mini-grid was not owned by and run for the benefit of the local community. Overall, we found that the mini-grid had a positive effect over background development, recovered some of its cost and charged a higher tariff than the national rate.