The number of binding sites for Axinella lectin (specific for D-galactose) on monkey erythrocytes containing schizonts of Plasmodium knowlesi was found to be reduced by about a third, from approximately 11 X 10(6) sites on normal erythrocytes to approximately 8 X 10(6) sites on parasitized erythrocytes. In contrast, only minor differences were found in the amounts of iodinatable external proteins. The results indicate that there is a general reduction of accessible sugars to which lectins may bind on parasitized erythrocytes.
Much has been written about the need for continuing education and training to enable life scientists and computer scientists to manage and exploit the different types of biological data now becoming available. Here we describe the development of an online programme that combines short training courses, so that those who require an educational programme can progress to complete a formal qualification. Although this flexible approach fits the needs of course participants, it does not fit easily within the organizational structures of a campus-based university.
Absorption experiments with washed membranes derived from erythrocytes infected with schizonts of Plasmodium knowlesi (PE) showed that the antigen responsible for agglutination of PE in SICA tests is firmly bound to membranes. Further experiments demonstrated that the number of antibody binding sites at the surface of parasitized erythrocytes can be measured using iodinated globulins. The conditions under which this could be achieved have been defined and a preliminary estimate of the number of high affinity surface antigen sites (greater than or equal to 10(4) per cell) has been made. Suggestions are given for further work to obtain a more accurate value.
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