SUMMARYThe present study was designed to investigate the effects of nicardipine on cadmium-induced Purkinje cell loss in cerebellum of rats. Animals were divided into three groups as control, cadmium and cadmium + nicardipine groups. Saline, cadmium sulphate (0,0021 mg/kg) and cadmium sulphate (0,002 1 mg/kg) + nicardipine (1 mg/kg) were intracortically administered respectively. All rats were observed for one week, but only the rats in the third group were injected nicardipine (10 n&kg/day, i.p.) during this period. One week later, all rats were perfused intracardially. Series of frontal sections from cerebella were stained with Thionin staining. Purkinje cells were counted under x400 magnification via a light microscope.Cadmium administration caused 25-35 % decrease in Purkinje cell density in Crus I, Crus II and Lobulus Simplex areas of cerebellum in cadmium group (pcO.05). The Purkinje cell density was 16-23 % less in nicardipine-treated group than control and cells were partially protected from toxic effects of cadmium significantly (pcO.05).These results suggest that cadmium has neurotoxic effects on Purkinje cells in cerebellum and nicardipine may protect neurons from cadmium-induced toxicity 6~0.05).
Sıçanlarda (Wistar albino) somatomotor kortekse kadmiyum sülfat (CdSO 4) verildikten sonra spesifik Nitrik Oksit Sentaz (iNOS) enzim inhibitörü olan Aminoguanidin (AG) ve NO öncüsü olan L-Arjinin (L-Arg)'in beyin ve beyincikteki toplam lipid ve protein miktarlarına etkisini tespit etmek üzere, sunulan çalışma planlandı. Bu amaçla, saf, kontrol, kadmiyum, kadmiyum+L-Arg ve kadmiyum+AG olmak üzere 7'şer sıçandan oluşan 5 grup oluşturuldu. Kadmiyum grubu sıçanlara 0,0021 mg/kg CdSO 4 sol hemisfere (bregmanın lateraline) intrakortikal yoldan enjekte edildi. Kontrol grubuna aynı hacimde serum fizyolojik verildi. Kadmiyum+AG grubuna günde iki kez olmak üzere 100 mg/ kg AG 15 gün süreyle intraperitoneal yoldan verildi. Kadmiyum+L-Arjinin grubuna da günde iki kez olmak üzere 1000 mg/kg L-Arjinin 15 gün süreyle intraperitoneal yoldan verildi. Saf grup ise hiçbir işleme tabi tutulmadı ve hiçbir madde verilmedi. Elde edilen sonuçlar, çalışılan diğer gruplara göre kadmiyumun toplam lipid ve protein miktarlarını düşürdüğünü ve toksik etkiye sahip olduğunu göstermektedir. Ayrıca, sunulan çalışma ile sıçanda kadmiyumun bu nörotoksik etkisinin, spesifik NOS inhibitörü olan AG tarafından azaltıldığına dair sonuçlar yeni bulgulardır. L-Arjininin kadmiyumdan kaynaklanan toplam lipid ve protein miktarlarındaki azalmayı arttırdığını ve bu nörotoksik etkide NO'nun da rol aldığını, elde ettiğimiz bulgular göstermektedir.
SUMMARYThe effects of the calcium channel blocker flunarizine on cadmium-induced Purkinje cell loss in the cerebellum of rats were investigated. Rats were divided into three groups: control, cadmium, and cadmium+flunarizine.Saline was administered intracortically to the first group, cadmium sulphate (0.0021 mg/kg) to the second group, and cadmium sulphate (0.0021 mg/kg) and flunarizine (1 mg/kg) to the third group. All rats were observed for 1 week, but only the rats in the third group were injected with flunarizine (10 mg/kg/day, i.p.) during this period. One week later, all rats were perfused intracardially.Series of frontal sections from the cerebellum were stained with thionin. Purkinje cells were counted at x 400 magnification under a light microscope. Purkinje cell density was 20.
Objective: Aluminium (Al) is quite abundant in nature and humans are frequently exposed to Al in daily life. Aluminium salts can exist in different forms and they may have toxic impacts on several tissues including brain. In this study, potential preventive effects of amino-guanidine (AG) (100 mg/kg, i.p.), an inducible nitric oxide synthase inhibitor, on neuron damage to be created by aluminium sulphate (3 mg/kg, i.c.v.) in cerebellar Purkinje cells were determined. Methods: 24 female Wistar albino rats were divided into 4 groups with 6 rats in each: Control (C), Sham (S), Aluminium sulphate (Al2(SO4)3), Aluminium sulphate + Amino-guanidine (Al2(SO4)3+AG). A single aluminium sulphate (3 mg/kg) dose dissolved in 0.9% NaCl was injected intracerebroventricularly to aluminium sulphate and aluminium sulphate + amino-guanidine groups at the beginning of experiments. Following aluminium sulphate injection, amino-guanidine (100 mg/kg) dissolved in distilled water was injected to aluminium sulphate + amino-guanidine group intraperiteonally for 15 days. Nothing was administered to control group, a single dose of 0.9% (3 mg/kg, i.c.v.) sodium chloride (NaCl) was administered to sham group at the beginning of experiments. Cerebellum tissues of the rats were removed 15 days after treatments and they were assessed histopathologically and stereologically. Results: Stereological optic fractionation method revealed cerebellar total number of Purkinje cells as 417615±16238,8 in control group; 378650±20171,6 in Sham group; 272945±15499,5 in Aluminium sulphate group; 324581±16324,8 in Aluminium sulphate + Amino-guanidine group. Conclusion:It was concluded based on present findings that amino-guanidine reduced aluminium induced Purkinje cell loss through nitric oxide synthase (NOS) inhibition.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.