This study was designed to evaluate the present biosecurity measures in broiler farms in Khartoum and to compare between the biosecurity practices followed in closed system and open system farms. The primary information that included numbers of broiler poultry in each farms, locations in the state and type of farms were obtained from Ministry of Agriculture and Animal Resources, Khartoum. Multi-stage cluster sampling method was used; collection of information was done at different levels (sites, farms and persons). A total of 45 broiler farms (13 closed and 32 open systems) were chosen from Khartoum, Khartoum North and Omdurman. Data were collected by using structured questionnaire. The respondents were farm owners, farm managers, veterinarians and workers. The results showed that the closed system had a higher level of biosecurity than the open system. 100% of the closed system practiced all in all out system when compared with 81.3% of the open system farms. The open system farms had less secure boundaries; 28.1% of the farms did not have fence when compared with 100% of the closed system farms. Only 2.2% of the farms had washing by disinfectants at the gates. The distance among the pens in each farm which was more than 100 m was 33.3%. Among the 45 farms, 87.5% disinfected the equipment before each production cycle. It was found that 88.9% of the farms collected dead chickens once daily. The results, also, showed that 57% of the farms did not use disinfectants in their foot dips of each pen and 84.4% had no warning signs for entrance of unauthorized people. Among these farms, 17.8% had control plan for vermin, 68.9% of the farms isolated the sick birds, and 26.7% were found to keep different species of birds other than poultry and 15.5% used to treat drinking water for poultry. It is concluded that the majority of the farms in were far from application of biosecurity measures.

This study was conducted to evaluate the current implementation level of Hazard Analysis and Critical Control Point (HACCP) prerequisites adopted in poultry meat production and processing in Khartoum State, Sudan. To achieve the goal, a cross sectional study was conducted. Data and samples were collected from January to September 2018 from 12 close system broiler slaughterhouses according to Non-Probability Multistage Cluster Sampling Method in Khartoum, Khartoum North (Bahri), and Omdurman localities (4 farms from each). A standardized questionnaire was conducted for slaughterhouses (12 for each) to investigate the current status of implemented HACCP prerequisites. One hundred and eighty swab samples were taken from different sites in the slaughterhouses’ halls (surfaces, workers’ hands, boots, water, and chillers) followed by 240 swab samples collected from broiler carcasses after 4 process steps (defeathering, evisceration, washing and chilling). After bacterial culturing, isolation, and identification, all collected Data were subjected to descriptive and analytical statistic such as Chi square and ANOVA using SPSS. Low level of good practices in slaughterhouses was shown regarding programmed documented personal hygiene staff training and qualification for responsibilities 50.0% (n=6), preventative maintenance 41.7% (n=5), and appropriate use of personal facilities 58.3% (n=7). In addition to that, 66.7% (n=8) of examined premises showed difficulty in cleaning, inadequate ventilation and 33.3% (n=4) showed lack of sanitary facilities (dispensers, personnel changing rooms, toilets, washing basins). Unhealthy practices and habits were observed in majority of workers during work such as not washing hands before entering production areas, smoking, and eating and drinking in processing areas, beside low level of medical check in two thirds of them 66.7% (n=8). Bacterial growth was shown in 60.0% (n=108) and 58.80% (n=141) of slaughterhouses’ halls samples and meat samples respectively. There was significant difference between state of growth of slaughterhouses’ halls sample and farm location (χ2=7.22 and P-value = 0. 027) while a high significant difference in association between state of growth of meat samples and slaughterhouses’ location (χ2=43.02 and P-value=0. 000) was revealed. The Gram positive and Gram negative isolates were mostly detected in workers’ hands 19.60% (n=56) and 4.36% (n=12) respectively. The difference between growth state of bacteria in slaughterhouses’ halls samples and sample sites was highly significant (χ2=30.92 and P-value=0.000) and the highest growth was shown in workers’ hands. On the other hand, most of Gram positive and Gram negative bacteria in processing steps were detected after defeathering 14.40% (n=41) and evisceration 2.49% (n=7) steps. However, there was no significant difference resulting from association of state of bacterial growth of poultry meat samples and different process steps (χ2=5.14 and P-value=0.162). Slaughterhouses’ halls samples and meat samples were found contaminated with harmful pathogenic bacteria such as Salmonella spp. 1.41% (n=4), Escherichia coli 9.51% )n=27(, Staphylococcus aureus 7.75% (n=22), Citrobacter frundi 1.41% (n=4), and Micrococcus kristanae 8.75% (n=24). In conclusion, effective adoption of HACCP PRPs and biosecurity measures in Khartoum State has not been given serious attention beside lack of knowledge, resources, and absence of official authority’s role. Absence of proper “Farm to Table” hygiene policy in broiler production negatively affects poultry meat hygiene and consequently threatens public health. Therefore, formulation of suitable procedures and regulations by official authorities for implementing HACCP PRPs and biosecurity measures are needed to ensure poultry meat hygiene from primary stages of production till the end product.

No abstract

This study was conducted to evaluate the level of adopted biosecurity measures in broiler farms based on manure bacterial load examination in Khartoum State, Sudan. To achieve the goal, a cross sectional study was conducted. Data and manure samples were collected from January to September 2018 from 12 close system broiler farms according to Non-probability Multistage Cluster Sampling Method in Khartoum, Khartoum North (Bahri), and Omdurman localities (4 for each). A standardized questionnaire was also conducted and used for data collection. A total of 12 manure samples were collected from one chosen broiler house in each farm to check the TVCs. All collected Data were subjected to descriptive and analytical statistic such as Chi square and ANOVA using SPSS. The Results of external biosecurity measures revealed that half of the total farms examined (50%) were at a distance between 500 m to 1km from the nearest poultry farm. The same percentage was obtained for no manure spreading from neighboring farms. However, frequent passage of other farms vehicles via near public road was reported in 50% of participating farms. The internal biosecurity results showed that all the examined farms (100%) prohibiting manure storage beside 75.0% of them remove the manure through the dirty area. Furthermore, workers protective wearing when contact waste and hygiene practicing after removing waste sometimes practiced by 75.0% of the participant farms. The mean of bacterial TVCs in manure samples was found below the cut-off level (6.01±0.78 cfu/g) with 95% confidence intervals of 4.48-7.37, 5.20-6.79, and 4.39-7.84 and mean TVC of 5.93±0.90, 6.00±0.50, and 6.11±1.08 cfu/g for Khartoum, Khartoum North, and Omdorman, respectively. No significant difference was obtained for log means of bacterial TVCs and farm location (F value=0.047 and P-value=0.955). In conclusion, despite that the bacterial counts in manure samples were below the cut-off level, poor hygienic practices were detected. Formulation of suitable procedures and regulations by official authorities for implementing biosecurity measures is recommended.

Computational model was designed for feeding systems of small dairy farms in Egypt under Mixed Farming System (MFS) (Crops/livestock). The present case study was selected from El-Beheira gov-