The DNA repair enzymes–heavy metal interaction is an interesting project that can help elucidate several diseases. The current study aims to assess the lead effect in the two DNA repair genes RAD-18 Arg302Gln (rs373572) and OGG1 Ser326Cys in Gasoline station workers. The output showed that work types were Gasoline supply worker with a high percentage (73.52%) than other groups (11.76% and 14.7%) for a maintenance worker and station employee, respectively, significant lead (p 0.010) increasing in station worker than the control group, and significant lead changes among work types groups (p 0.000), the station employee has a low level of lead than other groups, while the Gasoline supply worker has a higher level than other groups, the RAD 18 gene showed two polymorphisms Gln/Gln and Gln/Arg, and OGG1 showed two haplotypes, single and double haplotypes, non-significant association although of high frequent of Glu/Arg in the gasoline supply worker, and significant association of allele frequency, significant association with station worker that have two types of haplotypes (single and double haplotypes) while lack of tri-haplotypes which prepared in higher percentage in control group. The lead level according to RAD 18 genotyping show non-significant (p 0.454) elevation in Gln/Arg genotyping, and according to OGG 1 haplotype lead level was non-significant, changed (p 0.481) between single and double haplotypes. From these outputs, it can be concluded that the lead level is a significant elevation in gasoline station worker and it did not affect RA18 and OGG 1 genotyping, the RAD18 did not associate with workers while OGG strongly associated with them.
Neonatal isolation (NI) has detrimental consequences on the hippocampal neurons of rat neonates. It has been reported to enhance neuronal cell death and impair memory behaviors. We conducted this study to assess the effects of NI on hippocampal apoptosis and recognition memory impairment in the hippocampus of rat neonates. One group of male Wistar rat neonates exposed to NI; rat neonates reared with 1-hour neonatal isolation (NI) for eight consecutive days (P2-P9). On the other hand, the control group reared normally. Novel object recognition test (NOR) test used to evaluate the effects of NI on recognition memory impairment. On day 22 (P22), a TUNEL assay was done. NOR demonstrated that rat neonates who experienced NI had long-term memory deficits (P<0.01). TUNEL assay results showed that NI increased the number of TUNEL positive neurons in CA1, CA3, and DG subfields of the hippocampus (P<0.05, P<0.001, and P<0.001). The present results indicated that NI exerted apoptotic effect and induced recognition memory impairment in the rat neonate’s hippocampus.
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