DSSP is the most commonly used method to assign protein secondary structure. It is based on a hydrogen-bond definition with an energy cutoff. To assess whether hydrogen bonds defined in a parameter-free way may give more generality while preserving accuracy, we examine a series of hydrogen-bond definitions to assign secondary structure for a series of proteins. Assignment by the strongest-acceptor bifurcated definition with provision for unassigned donor hydrogens, termed the SABLE method, is found to match DSSP with 95% agreement. The small disagreement mainly occurs for helices, turns, and bends. While there is no absolute way to assign protein secondary structure, avoiding molecule-specific cutoff parameters should be advantageous in generalizing structure-assignment methods to any hydrogen-bonded system.
Aerospace electrical systems are continuing to increase their voltage levels to meet the on-board power demands of more-electric aircraft (MEA) where hydraulic and pneumatic systems are replaced with electrical equivalents. This trend will only continue as hybrid and all-electric aircraft are developed. These higher power demands require the use of higher voltages and as such it is essential to explore the behaviour of the insulation system in the aerospace environment. This insulation must operate in an environment where the operating and ambient temperatures range from 250°C to -65°C, the air pressure is around one tenth of that at ground level and where the levels of humidity and ozone vary rapidly. Understanding the impact of these variables on aircraft high voltage insulation systems is crucial in predicting their behaviour and lifetime. Our work with the IAGOS atmospheric dataset presents worst-case and typical flight environments for higher than expected cruising altitudes, and uses the findings to compare the relative rates of degradation of insulating materials at ground and cruising altitudes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.