In the present study, the effects of chitosan on erythrocyte malondialdehyde (MDA) and glutathione (GSH) levels and glutathione peroxidase (GSH-Px), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6PDH) enzyme activities in lead toxicity-induced rats were investigated. Twenty-eight male Wistar albino rats were divided into four groups of control (C), lead group (Pb group), lead + chitosan group (Pb + CS group), and chitosan group (CS group). Lead groups were administered 50 mg/kg lead acetate intraperitoneally (ip) for 5 days and chitosan groups were administered 200 mg/kg chitosan for 28 days via gavage. At the end of the study, lead levels were measured in the blood; MDA and GSH levels and GPx, GR, and G6PDH activities were measured in the erythrocyte. It was determined that, in parallel with the increase of full blood lead levels in the Pb group, erythrocyte MDA levels increased significantly, while GSH levels and GSH-Px, GR, and G6PDH activities decreased when compared to those in the C and CS groups (p ˂ 0.05). There was a statistically significant decrease in lead and MDA levels and GSH level and GSH-Px activity increased (p ˂ 0.05) in the Pb + CS group, where chitosan was administered as a protective agent in addition to lead, when compared to the Pb group. There were no differences between the Pb + CS group and the other three groups based on GR and G6PDH activities (p ˃ 0.05). No statistically significant difference was found between the C and CS groups based on the parameters of analysis (p ˃ 0.05). The findings of the present study demonstrated that lead increased oxidative stress by increasing free radical production in erythrocytes, and chitosan was effective in removing the lead from the circulation and enforced the antioxidant defense system.
Chitosan is a natural polymer with antioxidant and chelating properties. This study investigated the effects of chitosan on lead (Pb), malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), glutathione (GSH), ceruloplasmin concentrations and catalase (CAT) activity in the kidney tissue of the rats exposed to lead. 28 male Wistar albino rats were divided into four groups of eight each: control (C), lead group (Pb group), lead+ chitosan (Pb+CS group), and chitosan (CS group). The lead group was administered 50 mg/kg lead acetate intraperitoneally (ip) for 5 days, and the chitosan groups (CS+Pb and CS groups) were administered 200 mg/kg chitosan for 28 days via gavage. At the end of the study, Pb, MDA, 8-OHdG, ceruloplasmin, GSH concentrations and catalase activity were measured in the kidney tissue. In the Pb-treated groups when compared with the control group, Pb, MDA, 8-OHdG, ceruloplasmin concentrations were significantly increased, and GSH concentration and catalase activity were significantly decreased (p<0.05). Coadministration of chitosan with lead significantly decreased Pb, MDA, and ceruloplasmin levels and significantly increased CAT activity in the kidney tissue (p<0.05). There were no significant changes in GSH and 8-OHDG levels (p>0.05). The obtained results show that chitosan protects the kidney against leadinduced oxidative stress.
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