In the city of Magelang, Indonesia, the distribution of Dengue Haemorhagic Fever (DHF) cases tend to be clustered, ever changing along with human urbanization from 2014 to 2017. Although DHF cases have been less reported in the city of Magelang for the past 5 years, vector control measures by using insecticide space spraying, particularly permethrin, have been continuously performed. Current study aimed to detect kdr mutations associated with pyrethroid resistance in Ae . aegypti and to study possible association between insecticide resistance and DHF case distribution related to human urbanization. The study was a cross sectional study conducted in 3 sub-districts in the city of Magelang, Central Java, Indonesia. Eggs of Ae . aegypti collected from 195 sample households were reared and were tested for resistance to pyrethroids by using PCR. Primers AaSCF1 and AaSCR4, and primers AaSCF7 and AaSCR7 were used in detecting presence of mutation in VGSC IIS6 and IIIS6 gene, respectively. Fragments of amplified DNA were sequenced and were analyzed. Spatio-temporal using Standard Deviational Ellipse (SDE) was performed to obtain mapping of DHF case distribution trends. The total number of DHF case was 380 cases, with the most cases (158) occurred in 2015 and the least cases (66) reported in 2017. DHF case distribution was grouped into several clusters. SDE calculation demonstrated movement of DHF case in the direction to principal arterial road, suggesting link to urbanization. Gene sequencing demonstrated VGSC IIS6 gene mutation (S989P and V1016G) in Ae . aegypti collected from study areas, indicating resistance to permethrin. VGSC IIIS6 gene mutation was not found. Current study concluded that multiple kdr mutations associated with resistance to pyrethroid was detected in Ae . aegypti , and that human urbanization may have a role in the development of such resistance.
Tujuan: Demam Berdarah Dengue (DBD) adalah penyakit yang disebabkan oleh virus dengue, ditularkan melalui gigitan nyamuk Aedes sp. DBD di Indonesia merupakan salah satu penyakit endemis dengan angka kesakitan yang cenderung meningkat dari tahun ke tahun. Daerah terjangkit semakin meluas mencapai 400 kabupaten/kota dari 474 kabupaten/kota di Indonesia dan sering menimbulkan KLB. Sistem surveilans DBD dilaksanakan sebagai salah satu upaya pengendalian penyakit DBD di Indonesia, salah satu hal penting dalam sistem surveilans adalah pelaporan kasus DBD. Tujuan evaluasi sistem pelaporan kasus DBD adalah untuk mengetahui hal yang berkaitan dengan sistem pelaporan kasus DBD di Kota Magelang tahun 2016. Metode: Studi evaluasi menggunakan metode deskriptif analitik, pengumpulan data primer dilakukan pada lima puskesmas dan satu RS melalui wawancara menggunakan kuesioner dan observasional pada informan sebanyak tujuh orang. Variabel yang di evaluasi berupa ketepatan dan kelengkapan laporan kasus. Hasil: Laporan DBD dikirimkan RS ke Dinas Kesehatan Kota Magelang, puskesmas mendapatkan informasi kasus DBD dari Dinas Kesehatan dan selanjutnya dilakukan penyelidikan epidemiologi. Kelengkapan laporan DBD dari RS ke Dinas Kesehatan sebesar 100% dengan ketepatan sebesar 51,28%. Keterlambatan pelaporan kasus DBD oleh RS disebabkan keterlambatan informasi diagnosis DBD dari ruang perawatan ke petugas surveilans RS, selain itu dikarenakan keterbatasan petugas surveilans RS. Laporan kasus DBD tidak ditembuskan ke puskesmas disebabkan ketidaktahuan petugas surveilans tentang alur pelaporan kasus DBD. Pelaporan kasus DBD oleh puskesmas tidak dapat dihitung baik kelengkapan maupun ketepatan, karena puskesmas hanya menerima informasi laporan kasus DBD dari RS melalui Dinas Kesehatan. Simpulan: Laporan kasus DBD di wilayah Kota Magelang hanya bersumber dari RS dan dikirimkan ke Dinas Kesehatan, kelengkapan laporan baik sedangkan ketepatan belum optimal, petugas surveilans RS tidak mengetahui alur pelaporan kasus DBD. Studi ini merekomendasikan untuk meningkatkan pengetahuan petugas tentang sistem pelaporan kasus DBD, termasuk ketepatan dan kelengkapan serta alur pelaporan dan kasus DBD.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.