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There are several scientific works has been done with alternative medicines in preventive measurement of diabetes mellitus. The present study was designed to evaluated in-vitro antioxidant activity of newly developed three ayurvedic formulations of Alternanthera philoxeroides and quantifies possible groups of phyto-constituents present in it. The prepared ayurvedic formulations are 'swarasa kalpana', 'hima kalpana', and 'phanta kalpana', which were mentioned in the ayurvedic classical book 'Sarangadhar Samhita'. This is first effort to explore the potentially of these formulations by in-vitro antioxidant activity as well as level of antioxidant groups (total flavonoid, total flavonoid etc.). The Graph pad prism (Version-3) software evaluates the interrelationship between the formulations as well as the analyzed parameters. It can be concluded that the antioxidant activity might be responsible for the presence of phenolic, flavonoid and other phytoconstituents.

The key purpose of this experiment was to evaluate the thrombolytic, antioxidant, membrane stabilizing and antimicrobial potentials of crude ethanol extracts (CEE) of whole plant, organic and aqueous soluble fractions (OF & AQSF). CEE showed the highest (44.63%) clot lysis activity compared to streptokinase (64.35%). In DPPH study, petroleum ether soluble fraction (PSF) has exhibited IC50 of 18.83 μg/mL while the standard ascorbic acid was 2.48 µg/mL. AQSF profoundly inhibited the lysis of erythrocytes (66.20%) which was insignificantly different (P>0.05) to acetylsalicylic acid (71.98%), the reference. However, AQSF showed a significantly stronger level of protection against heat-induced hemolysis (64.80%) as compared with the acetylsalicylic acid (78.90%). CEE, OF and AQSF have displayed reasonable growth of inhibition of tested bacteria compared to negative control and standard drug (77.50 mg of GAE/g).

Introduction: Diospyros melanoxylon Roxb. is traditionally used medicinal plant. Previously, we confirmed the better antiproliferative and antidiabetic activity of ethyl acetate fractions (EAF) obtained from D. Melanoxylon. To assessment the hypothesis if nanoparticle-encapsulated fraction could progress bioactivity. Materials and Methods: We prepared nanoparticle encapsulation based on poly (lactide-co-glycolide) (PLGA) and confirmed encapsulation by scanning electron microscopy. Sub EAF (SEAF) and nanoparticles of sub ethyl acetate (NSEAF) were characterized for their antiproliferative and antidiabetic activity. Antiproliferative activity was performed using the methylene blue assay. Antidiabetic activity was performed on the basis of α-amylase and α-glucosidase assay. Results and Discussion: Comparatively, NSEAF showed better activity on different cancerous cell lines HCT116 (inhibitory concentration 50% [IC 50 ] 32.89 ± 1.71 µg/ml), MCF-7 (IC 50 36.13 ± 0.96 µg/ml) and PC-3 (IC 50 32.39 ± 1.91 µg/ml), and antidiabetic activity on the basis of α-amylase (IC 50 35.62 ± 2.56 µg/ml), and α-glucosidase (IC 50 73.52 ± 1.13 µg/ml) action at a dose-dependent manner. It is also demonstrated that NSEAF exerted very low toxic effect against normal cell lines. Conclusion: For this reason, NSEAF studied in the present research may be considered as possible future drug candidates for the treatment and management of cancer and diabetes.

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