Background: Fructo-oligosaccharides (FOS) are prebiotic agents with immunomodulatory effects involving improvement of the intestinal microbiota and metabolome. In this study, we investigated the cellular mechanisms through which FOS modulate intestinal antigen-specific CD4+ T cell responses in food allergy, using OVA23-3 mice. Methods: OVA23-3 mice were fed an experimental diet containing either ovalbumin (OVA) or OVA and FOS for 1 week. Body weight and mucosal mast cell protease 1 in the serum were measured as the indicator of intestinal inflammation. Single-cell suspensions were prepared from intestinal and systemic lymphoid tissues for cellular analysis. Cytokine production was measured by ELISA. Activation markers and intracellular cytokines in CD4+ T cells were analyzed by flow cytometry. Activated CD4+ T cells were purified to examine cytokine production. Results: Dietary intake of FOS provided moderate protection from the intestinal inflammation induced by the OVA-containing diet. FOS significantly reduced food allergy-induced Th2 cytokine responses in intestinal tissues but not in systemic tissues. FOS decreased OVA diet-induced IFN-γ+IL-4+ double-positive CD4+ T cells and early-activated CD45RBhighCD69+CD4+ T cells in the mesenteric lymph nodes. Furthermore, we confirmed that these CD45RBhighCD69+CD4+ T cells are able to produce high levels of IFN-γ and moderate level of IL-4, IL-10, and IL-13. Conclusions: Dietary intake of FOS during the development of food allergy attenuates the induction of intestinal Th2 cytokine responses by regulating early activation of naïve CD4+ T cells, which produce both Th1 and Th2 cytokines. Our results suggest FOS might be a potential food agent for the prevention of food allergy by modulating oral sensitization to food antigens.
The sidelobe level at tilts around 30-40 degrees in both the E and H planes due to a tapered excitation of units of 2×2 radiation slots is suppressed by introducing slit layers over a corporate-feed waveguide slot array antenna. The slit layers act as averaging the excitation of the adjacent radiating slots for sidelobe suppression in both planes. A 16×16-element array in the 70 GHz band is fabricated. At the design frequency, the sidelobe levels at tilts around 30-40 degrees are suppressed from −25.4 dB to −31.3 dB in the E-plane and from −27.1 dB to −38.9 dB in the H-plane simultaneously as confirmed by measurements. They are suppressed over the desired range of 71.0-76.0 GHz frequencies, compared to the conventional antenna.
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