Ovarian cancer is the most lethal gynecologic malignancy. Recently, several molecularly targeted anticancer agents have been developed for ovarian cancer; however, its prognosis remains extremely poor. The development of molecularly targeted therapy, as well as companion diagnostics, is required to improve outcomes for patients with ovarian cancer. In this study, to identify microRNAs (miRNAs) involved in the progression of ovarian cancer we analyzed serum miRNAs in patients with ovarian cancer using miRNA array and quantitative RT‐PCR and examined the anticancer properties of miRNA expression in ovarian cancer cells. In patients with ovarian cancer, high amount of miR‐135a‐3p in serum samples was significantly associated with favorable clinical prognosis. The amount of miR‐135a‐3p was significantly decreased in patients with ovarian cancer compared with patients with ovarian cysts or normal ovaries. In SKOV‐3 and ES‐2 human ovarian cancer cells, enhanced expression of miR‐135a‐3p induced drug sensitivity to cisplatin and paclitaxel and suppressed cell proliferation and xenograft tumor growth. These findings suggest that miR‐135a‐3p may be considered as a biomarker and a therapeutic agent in ovarian cancer.
Introduction: Power morcellator is useful as a surgical instrument for shredding a specimen during laparoscopic surgery; however, because this technology requires a widened porthole in the abdominal wall, we consider that it has scope for improvement in terms of esthetic outcome and perspective on pain. In addition, at the time of transvaginal removal of the shredded specimen, we often cannot have enough space to perform the removal because the vaginal cavity is narrow and deep; alternatively, it may be difficult to perform owing to the extremely large specimen present. We report here a case of transvaginal in-bag morcellation. Methods: A single hospital observational study that involved patients who underwent laparoscopic hysterectomy was conducted. A folded isolation bag (3M Steri-Drape Isolation Bag) was introduced into the peritoneal cavity, and the specimen was placed in the bag. The mouth of the bag was guided to the vaginal stump and then exteriorized. A smallsized wound retractor, along with the bag, was set at the vaginal cavity, and the hole was covered with a sterile glove. A 5-mm trocar was inserted into the glove for the endoscope, and a morcellator was inserted into the cavity via the glove under observation. We performed transvaginal in-bag morcellation without spillage and dissemination of unwanted cells and tissues. Conclusion: Transvaginal in-bag morcellation performed with our new technique requires neither a widened porthole nor lacerations of the vaginal wall and thus may prove beneficial for esthetic outcome and reducing pain.
Ovarian clear cell borderline tumors are extremely rare, accounting for less than 1% of all borderline ovarian malignancies. The preoperative diagnosis of ovarian borderline malignancy is difficult in some cases, and although the number of cases diagnosed incidentally after laparoscopy is increasing, the optimal treatment for each histological type is unclear. In this case report, we describe a 65-year-old woman who was diagnosed with a cystic ovarian clear cell borderline malignancy without adenofibromatous components after laparoscopy. She presented at our hospital due to an abnormal medical examination, and an MRI revealed a polycystic lesion in the left appendage area. No obvious malignant findings were observed, and laparoscopic bilateral salpingo-oophorectomy was performed. The pathology was determined to be cystic ovarian clear cell borderline malignancy. No additional postoperative treatment was given, and the patient was carefully monitored and has not experienced postoperative recurrence for 1.5 years.
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