Objective:The objective of present research work is to study in vitro metabolism of risperidone on isolated microsomes of rat liver. Methods: Its biotransformation was examined using isolated microsomes derived from rat livers. Their separation was accomplished on Jasco RP-HPLC C-18 column (250mm x 4.6mm x 5µm) using methanol: 50mM Potassium dihydrogen phosphate (50:50 %v/v) mobile phase, detected at a wavelength of 280nm. Results: In vitro metabolism of RIS was investigated using the cytochrome P450 (CYP450) enzymes supplemented with an NADPH-generating system. Incubation of RIS with rat liver microsomes resulted in the formation of 2 major hydroxy metabolites. Conclusion: Formation of all the metabolites is correlated with Phase I biotransformation CYP enzyme subfamilies. The method was extended to LC-MS for further characterization of the metabolites using standards and the probable metabolites were proposed.
<p>Benthic-pelagic coupling is responsible for the sudden appearance and disappearance of many coastal plankton blooms. Whether this signature is also reflected in <em>p</em>CO<sub>2</sub> and whether the processes involved are important for the carbon fluxes in the coastal ocean is unclear. To address these questions, we use an ecosystem model that accounts for benthic-pelagic coupling of three different functional phytoplankton groups. Coupled with the water column model GOTM, we investigate the air-sea CO<sub>2</sub> fluxes in the Baltic Sea and compared them with observations. We show that the variability is very well captured by the model. The relative importance of the life cycle processes in regulating carbon fluxes is demonstrated.</p>
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