Received for piililication Ortolicr 19. 19fiABSTRAc:T.-EXperimentS were made to test the influence of the pH of the fixing fluid (ranging from 1.0-8.1) and that of the chromating fluid (1.65-7.8) on subsequent silver impregnation. Brains of adult monkeys, cats, dogs, rabbits, guinea pigs, rats and mice were fixed by the pulsating-perfusion method of Haushalter and Bertram (1955), after first washing out the blood with saline-acacia solution of the same pH as that of the 10% formol-saline-acacia used for fixation. The brains were sliced to 3 mm thickness and the slices further fixed 1-2 days in 10% formalin with its pH adjusted to that of the preceding fixing fluid. Chromation for 1 day followed, with acidified ZnCrO,
colorless solid which after recrystallization from methyl alcohol melted at 64°. The filtrate, on suitable treatment, yielded more of the same substance, the total yield being about 60%. It is much more easily obtained by direct addition of dimethyl malonate to benzalacetone. Thus a methyl alcoholic solution containing 2.4 g. of ketone and 3 g. of ester was allowed to stand at room temperature for several days during which it was kept alkaline by successive additions of a few drops of a dilute solution of sodium methylate. On evaporation it deposited a pale yellow solid, which became colorless on recrystallization. When the substance was heated alone or mixed with the product from the cyano ester the melting point was 64°.
Untersuchungen an gebrauchten HycLangzeit-PI raulikolen auf Rapsolbasis aus einer axisstudie *
K. V o s r n a n n , Barbara S a n d n e r , M. S p i l k e r u n d H . I h r i g * * Institut fur Chemie und Physik der Fette der Bundesanstalt fur Getreide-, Kartoffel-und Fettforschung, Miinster, und Fuchs Mineralolwerke GrnbH. Mannheirn
In the Physical Review for the year 1957, as a sample, multiple authorship appears with greatest relative frequency in communications from government, academic, and industrial laboratories, in that order. For the three types, it appears that research is conducted on the average by groups and not by individuals. These conclusions are at variance with premises assumed recently by R. S. Uhrbrock (1).
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