Vitamin A Deficiency (VAD) is associated with significant morbidity and mortality from common childhood infections and is the world's leading preventable cause of childhood blindness. Studies showed that carotenoid is one of the promissed vitamin A sources. However the studies on carotenoid from yellow banana peel and its potential as a natural source of vitamin A has not been widely reported. This study was conducted to measure the blood serum retinol levels of rats after administration of β-carotene from yellow ambon banana peel. This was an experimental study with post test only control group design, with sample size of 18 rats with age 1 month, devided into 3 groups. The β-carotene dose administered based on the dose of red capsules vitamin A are (200,000 doses SI) for toddlers aged 12-59 months. Serum retinol levels were measured using a spectrophotometer according metide. This present study showed that the blood serum level in group treated with of β-carotene from yellow ambon banana peel (28.35 ± 1.61 mg/dL), was significantly different (p < 0.05) from that of control group (22.08 ± 1.35 mg/dL). The β-carotene from yellow ambon banana peel are potential as provitamin A.
Background: Carotenoid pigment is one of the promising sources of provitamin A because it has been shown to have capacity to be converted into vitamin A by the body. This present study aimed at evaluating the antioxidant activity in vitro using the DPPH and assessing the encapsulation of the crude carotenoid pigment extracted from Musa parasidiaca sapientum peel.Method: The total vitamin A carotenoid in the banana peel was assessed using the double beam Varian Cary 50 spectrophotometer at 470 nm. Then it was converted to microgram per gram of the banana peel using the NAS-NRC equation. The extraction was conducted using acetone. The antioxidant activity was assessed using DPPH (1.1-difenil-2-dipikrilhidrazil). The antioxidant activity of carotenoid crude extract was compared to that of marker â-karoten antioxidant (E-Merck, No. 1.02236). IC50 values were calculated using the regression formula. The dried carotenoid crude extract was encapsulated with dextrin filler.Result: The water content level of ambon banana was 50.68 % ± 3.35 %. The peel of the yellow ambon banana has a total carotenoid of 6.203 ± 0.004 μg/g. The converted carotenoid vitamin A was 124.06 ± 0.08 IU. The IC50 value of the yellow ambon banana crude extract was 2350.3 ppm meaning higher than the marker â-caroten (565.76 ppm). The encapsulation technique increased the pigment stabilization of the yellow ambon banana which will be used for vitamin A supplement.Conclusion: The crude extract of carotenoid pigment of the yellow ambon banana peel has an antioxidant potential although it is less efficient compared to that of â-carotene. The IC50 value of the crude extract of carotenoid pigment of the yellow banana ambon is 2350.3 ppm, higher than marker â -caroten (Sains Medika, 4(1):78-88).
AbstrakPemanfaatan kulit pisang akibat peningkatan konsumsi pisang ambon perlu dilakukan, salah satunya sebagai alternatif sumber vitamin A alami (SUVITAL) untuk mengatasi kasus kekurangan vitamin A (KVA). Potensi karotenoid kulit pisang ambon sebagai SUVITAL belum banyak diteliti. Penelitian ini bertujuan menguji aktivitas antioksidan dan toksisitas terhadap hati dan ginjal dari drop vitamin A karotenoid kulit pisang ambon (DKKP) dibanding dengan β karoten (DBKM) pada kelinci (Oryctolagus cuniculus) galur New Zealand White. DKKP dan DBKM dibuat sesuai formula vitamin A per 50.000 IU/mL. Penelitian ini dilakukan di Laboratorium Biologi Fakultas Kedokteran Universitas Islam Sultan Agung selama September-Oktober 2015. Sebanyak 12 ekor kelinci jantan dibagi menjadi 3 kelompok: kontrol, DKKP, dan DBKM. Drop diberikan per oral sebanyak 5 mL/ekor/ hari selama 7 hari. Efek antioksidan diuji berdasar atas kadar malondialdehyde (MDA) dan retinol serum darah, sedangkan toksisitas terhadap hati dan ginjal ditentukan berdasar derajat kerusakan hepatosit dan sel epitel tubulus proksimal ginjal. Hasil uji LSD menunjukkan bahwa kadar MDA dan retinol serum darah kelinci yang diberi DKKP tidak berbeda signifikan (p>0,05) dibanding dengan DBKM, demikian juga dengan skor kerusakan hepatosit dan sel epitel tubulus proksimal ginjal. Aktivitas antioksidan drop vitamin A dari karotenoid kulit pisang ambon sama dengan drop β-karoten murni. Drop vitamin A dari karotenoid kulit pisang tidak toksik terhadap histopatologi hati dan tubulus proksimal ginjal. The increased consumption of ambon banana has resulted in increased amount of banana peel; which opens an opportunity to use them as a natural vitamin A alternative source to prevent vitamin A deficiency (VAD).The potential use of banana peel as a vitamin A supplement has not been much investigated. This study was conducted to measure the antioxidant activities and toxicity of vitamin A drop from carotenoids of Ambon banana peel (DKKP) in comparison with the vitamin A drop containing pure β-carotene (DBKM) on New Zealand White rabbits' liver and kidney (Oryctolagus cuniculus) . The DKKP and DBKM were formulated according to the formula of vitamin A per 50,000 IU/ml. Twelve male rabbits were divided into 3 groups, i.e., control, DKKP, and DBKM. DKKP and DKKP groups received 5 ml/day oral dose for 7 days. Malondialdehyde (MDA) and retinol from blood serum were measured as the parameters of antioxidant activities while the hepatocyte and proximal tubule epithelium damage score were used to detect toxicity in liver and kidney. LSD test results showed that the levels of MDA and retinol in the blood serum of the DKKP group were not significantly different (p> 0.05) from those of the DBKM group. A similar situation was also seen for the hepatocyte damage score of the liver and proximal tubular epithelial cells of kidney. The antioxidant activities of vitamin A drop from banana peel carotenoid was equal to that of the vitamin A drop from pure β-carotene. Vitamin A drop from carotenoid of banana ...
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