The present study involved collection of 30 Al-Ramady General Hospital sewage, five samples for each 15 days from the treatment unit for the period October 2014 to February 2015 in triplicates. Six bacterial isolates were identified: Aeromonashydrophila, Aeromonassobria, Escherichia coli, Klebsiella pneumonae, Staphlyococcushominis and Staphylococcus aureus. All these isolates were tested for their ability to remove lead from its aqueous solutions. Significant differences (P< 0.05) were found among bioremoval capacity which were as follows 11.
The effect of metabolic status on lead bioremoval byKlebsiella pneumoniae were tested. The results revealed a significant differences (P<0.05) between live and dead (killed by heat) cellsas the bioremoval reached 57.0 ± 3.4 and 21.6 ± 2.5, respectively. Bioremoved lead ions were recovered (P<0.05) using three different washing solutions, 0.1M EDTA was the most efficient since it recovered about 63.9 ± 5.6 mg/ml, while Na2CO3 was able to recover 35.9 ± 2.6 mg/ml; whereas 0.1M HCl recovered 21.8 ± 2.6 mg/ml. genomic DNA was extracted from different isolates of K. pneumoniae in order to study the relationship between genotype using BOX-PCR and bioremoval of lead. Three clusters were detected C1, C2, and C3 with similarity percentages reached 52, 60, and 100%. What's more, bioremoval did not depend on particular genotype
The ability of acid supernatants of Lactobacillus acidophilus isolated from a vinegar were tested against methicillin resistant Staphylococcus aureus (MRSA) with lipolytic activity isolated from acne pimple. Results revealed a significant effect of cell free supernatant on MRSA isolates in comparison with control. Furthermore, subinhibitory concentrations of acid supernatants found to be very effective in inhibiting the production of lipase from biofilm and planktonic cells of MRSA isolates. What's more, acid supernatants of L. acidophilus showed inhibiting effect on the crude lipase in egg yolk agar plates.
One hundred and sixty five mid stream urine specimens were collected from outpatients presented with urinary tract infections (UTI). The results showed the dominance of Escherichia coli over other causative agents. Antibiotic sensitivity test was carried out to E. coli isolates. Thence, the isolate that developed the highest multidrug resistance was chosen for further studies. Moreover, five Lactobacillus isolates comprising L. acidophilus L1 and L. acidophilus L2 were isolated from yogurt and vinegar, respectively, L. plantarum L3 and L. plantarum L5 from saliva and raw milk, respectively, while L. fermentum L4 was isolated from vagina. Cup assay method was employed to investigate the inhibitory (antagonistic) activity of lactobacilli isolates against E. coli A99 on MRS agar. Results showed that L. acidophilus L1 developed the highest activity. The cell free supernatant of lactobacilli developed the same activity. L. acidophilus L1 supernatant showed the highest inhibition activity. The present study also revealed this activity in vivo by injecting a group of mice with L. acidophilus L1 suspension or its infiltrate 30 min after injecting the E. coli A99 intraurethrally and the histopathological sections revealed the disappearance of inflammation signs caused by E. coli A99 when it was injected alone.
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