Aim:The aim of this ex vivo study was to compare the antibacterial activity of total-etch and self-etch adhesive systems against Streptococcus mutans, Lactobacillus acidophilus, and Actinomyces viscosus through disk diffusion method.Materials and Methods:The antibacterial effects of Single Bond (SB) and Adper Prompt (AP) and aqueous solution of chlorhexidine 0.2% (positive control) were tested against standard strain of S. mutans, L. acidophilus, and A. viscosus using the disk diffusion method. The diameters of inhibition zones were measured in millimeters. Data was analyzed using Kruskal-Wallis test. Mann-Whitney U test was used for pairwise comparison.Result:Of all the materials tested, AP showed the maximum inhibitory action against S. mutans and L. acidophilus. Aqueous solution of chlorhexidine 0.2% showed the maximum inhibitory action against A. viscosus. Very minimal antibacterial effect was noted for SB.Conclusion:The antibacterial effects observed for the tested different dentin bonding systems may be related to the acidic nature of the materials.

Aim and Objectives: In dentistry, base metal casting alloys are extensively used for the fabrication of inlays, onlays, crowns, bridges, partial dentures, etc. During the casting of these alloys, excess amount of material used than needed will be collected as sprue buttons at the end, which is either added to the fresh alloy during casting and reused or disposed of. Materials and Methods: The aim of the present in vitro experimental study was to investigate the effect of the complete recasting of four commercially available cobalt-chromium (Co-Cr) and nickel-chromium (Ni-Cr) base metal casting alloys on their cytotoxicity. During the study, four groups of alloys were subjected to complete recasting up to twenty times without the addition of new alloy. The cytotoxicity assessment of the selected alloys after recasting (Co-Cr and Ni-Cr alloys) was carried out using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: The results indicated that Co-Cr alloys exhibit superior cell viability compared to Ni-Cr alloys, and cytotoxic potential of the alloys increased with repeated casting and led to increased cell death. The recasting of alloys in the present study did not show high cytotoxicity even after the 20 th recasting. Conclusion: From the results of the present study, it can be concluded that the alloys generated as a waste in the dental laboratory can be safely reused up to five times or at least once before they can be disposed, which reduces the cost of the treatment and also helps in conserving the natural resources.

Aim:To investigate the effect of bleaching agents having a neutral pH on the surface of mineral trioxide aggregate (MTA) used as a coronal seal material for nonvital bleaching, beneath the bleaching agent, with the help of energy dispersive X-ray microanalysis and scanning electron microscopy (SEM).Materials and Methods:Six samples of plastic tubes filled with white MTA (Angelus white) were kept in 100% humidity for 21 days. Each sample was divided into 2 and made into 12 samples. These were then divided into three groups. Group A was exposed to Opalescence Boost 40% hydrogen peroxide (HP) (Ultradent). Group B to Opalescence 10% carbamide peroxide (Ultradent) and Group C (control group) not exposed to any bleaching agent. After recommended period of exposure to bleaching agents according to manufacturers’ instructions, the samples were observed under SEM with an energy dispersive X-ray microanalysis system (JSM-6380 LA).Results:There were no relevant changes in color and no statistically significant surface structure changes of the MTA in both the experimental groups.Conclusion:The present findings suggest that even high concentration HP containing bleaching agents with neutral pH can be used on the surface of MTA without causing structural changes. The superior sealing ability of MTA and the high alkalinity would prevent cervical resorption postbleaching.

The aim of this study was to quantify the marginal leakage of two glass-ionomer cements using spectrophotometer. Method and materials: Standardized class V cavities were prepared on 60 extracted human maxillary permanent first premolars, randomly assigned to two groups of 30 teeth each, group A and group B. Group A was restored with Zirconomer and group B was restored with GC Fuji IX Extra. Teeth were subjected to thermocycling and stained with methylene blue. The microleakage was quantified spectrophotometrically and the data were statistically analyzed with Student t test. Results: Restorations of both the materials showed some microeakage in Class V cavities and there were no significant differences statistically. Conclusion: The sealing ability of GC Fuji IX Extra was much better than Zirconomer and GC Fuji IX Extra exhibit better shade matching compared to Zirconomer at cervical area of the teeth.

Background: Calcium silicate-based cements or bioceramics have revolutionized and simplified pulp-capping procedures due to its versatility and accessibility. Aim: This study aimed to assess the shear bond strength (SBS) of a bioceramic material to composite resin at two aging periods and with three universal adhesives. Materials and Methods: Forty-eight samples of Biodentine with a height of 2 mm and internal diameter of 4 mm were prepared. After 12 min of mixing, 24 samples were randomly selected and divided into four subgroups: Group I: Single Bond Universal, Group II: Palfique Universal Bond, Group III: G-Premio BOND, and Group IV: Control. Composite resin was restored over Biodentine after the application of universal adhesives using a cylindrical plastic mold of 2 mm height and 2 mm internal diameter. This process was repeated 24 h after mixing on the remaining 24 samples of Biodentine. The universal testing machine was used to measure the SBS of the fractured specimens, followed by stereomicroscopic evaluation. One-way ANOVA test and Bonferroni post hoc test were used to statistically analyze the data. Results: Highest SBS values were observed in Group III at 12 min and 24 h of the setting of Biodentine. Conclusions: Compared to the 12-min group, SBS was higher at 24 h. Group III exhibited higher bond strength than other subgroups.

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