Isotomurus maculatus (Collembola, Isotomidae) is a springtail with a large distribution. This species has been introduced to the sub-Antarctic Prince Edward Islands. In this study, the mitogenome of I. maculatus was reconstructed. The total length of the mitochondrial genome is 15,263 bp and comprises 13 protein-coding genes, 22 tRNAs, and two rRNAs. The nucleotide composition is 34.4% adenine, 29.2% thymine, 15.1% guanine, and 21.3% cytosine. A Bayesian phylogenetic tree indicates that I. maculatus clusters as the sister taxon to the genus Cryptopygus, with high statistical support.
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