Overexpression of SERCA2a protein results in a positive inotropic effect under baseline conditions remaining preserved under pressure overload without affecting mortality. Therefore therapeutic transfer of SERCA2a may become a potential approach for gene therapy of congestive heart failure. Moreover, transgenic SERCA2a rats will be useful for studies of long-term SERCA2a overexpression in further cardiovascular disease models.
Nitrate uptake was followed continuously in cultures of Chlorella sorokiniana using ionsensitive electrodes. During the lifecycle of the synchronous cell cultures, a drastic increase occurred in the first hour after the onset of the light. Nitrate uptake rate was shown to be dependent on illumination intensity, nitrate concentration, and temperature. These results point to an energy-linked uptake process. From the different timecourses of nitrate uptake rate and nitrate reductase activation, one can conclude that the increased nitrate reductase activity after light onset is regulated via nitrate uptake. Further evidence for a regulation in this direction is shown by the action of ammonium on nitrate uptake and nitrate reductase activity. The results are discussed in terms of the regulation of the nitrate consuming system.
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