Combined music therapy and sufentanil improves intravenous patient-controlled analgesia effects compared with sufentanil alone after lung cancer surgery. Lower doses of sufentanil could be administered to more effectively improve patients' cardiovascular parameters.
MicroRNA (miR)-221 plays an essential role in the epithelial-mesenchymal transition (EMT). High mobility group AT-hook 2 (HMGA2), is a key regulator of EMT. However, the role of miR‑221 in pulmonary fibrosis, and the association between miR‑221 and HMGA2 remain largely unknown. For this purpose, we examined the expression of miR‑221 and HMGA2 in human idiopathic pulmonary fibrosis (IPF) tissues and pulmonary cells, namely the adenocarcinoma A549 and human bronchial epithelium (HBE) cell lines, and found that the expression of miR‑221 was inhibited in both tissues and cells whereas high mRNA and protein expression of HMGA2 was observed. Additionally, transforming growth factor‑β1 (TGF‑β1) induced the EMT, characterized by the upregulated expression of the mesenchymal markers, namely N‑cadherin, vimentin, α‑smooth muscle actin, collagen I and collagen III, and the downregulated expression of the epithelial marker E-cadherin in A549 and HBE cells. We then performed transfection with miR‑221 mimics, and found that the expression of phosphorylated-Smad3 in miR‑221‑overexpressing cells was significantly downregulated, compared with that in the TGF‑β1-treated cells without transfection. Furthermore, the overexpression of miR‑221 decreased the expression of HMGA2, suppressed the EMT, and inhibited the proliferation of A549 and HBE cells. HMGA2 was directly targeted by miR‑221 which was confirmed by the dual-luciferase reporter gene assay. Finally, a mouse model of bleomycin (BLM)‑induced pulmonary fibrosis was used to confirm the effect of miR‑221 on EMT. Hematoxylin and eosin staining showed that BLM induced thicker alveolar walls and more collagen deposition, whereas miR‑221 treatment reduced lung fibrosis and the tissues exhibited thinner alveolar walls and normal lung alveoli. Furthermore, the EMT process was suppressed following miR‑221 injection. Taken together, these findings sugest that miR‑221 targets HMGA2 to inhibit BLM‑induced pulmonary fibrosis through the TGF‑β1/Smad3 signaling pathway.
Introduction: Pregnancy and childbirth are highly challenging, as it is an event of significant psychological, social and emotional change for women that may predispose them to stress and anxiety. Music therapy is emerging with increasing popularity as an additional treatment for ameliorating stress, anxiety and labor pain. This study aimed to examine the effect of music interventions on maternal perinatal mental health and childbirth outcomes to establish comprehensive evidence for clinical decision-making. Methods: This study is a systematic review and meta-analysis. Results: Twenty-six randomized controlled trials (RCTs) involving 3,969 women included in this review. The overall effects indicated that anxiety levels in women in the music group were significantly lower than in the usual care group (SMD =-4.17, 95% CI:-5.13 to-3.20). The pooled results also indicate that music interventions could significantly reduce depressive symptoms (SMD =-2.66, 95% CI:-4.98 to-0.34). The music interventions also tended to reduce women's stress, but without significantly statistical differences (Z=1.84, P=0.07). Eight studies reported the effects of music interventions on the physical outcomes of pain and blood pressure and pain. Music interventions could significantly reduce pain intensity (SMD =-1.43, 95% CI:-2.67 to-0.18, blood pressure: systolic BP (SMD =-2.23, 95% CI:-3.76 to-0.70), and diastolic BP (SMD =-1.80, 95% CI:-3.00 to-0.61), and reduced heart rate vital signs (SMD =-3.33, 95% CI:-4.36 to-2.30). Five studies reported the effects of music interventions on women's satisfaction and quality of life, and found that music interventions significantly improved these outcomes (SMD =2.86, 95% CI: 1.66 to 4.05). In terms of child outcomes, the music interventions positively stimulated fetal movement (SMD =1.62, 95% CI: 0.05 to 3.19) and accelerations (SMD =1.08, 95% CI: 0.74 to 1.42). Conclusions: These results suggest that music interventions may have the potential to reduce obstetrical complications, so that application of music interventions across all three trimesters and during labor may be advisable in routine obstetric practice.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.