In many eukaryotes, cytokinesis requires the assembly and constriction of an actomyosin-based contractile ring. Despite the central role of this ring in cytokinesis, the mechanism of F-actin assembly and accumulation in the ring is not fully understood. In this paper, we investigate the mechanism of F-actin assembly during cytokinesis in Schizosaccharomyces pombe using lifeact as a probe to monitor actin dynamics. Previous work has shown that F-actin in the actomyosin ring is assembled de novo at the division site. Surprisingly, we find that a significant fraction of F-actin in the ring was recruited from formin-Cdc12p nucleated long actin cables that were generated at multiple nonmedial locations and incorporated into the ring by a combination of myosin II and myosin V activities. Our results, together with findings in animal cells, suggest that de novo F-actin assembly at the division site and directed transport of F-actin cables assembled elsewhere can contribute to ring assembly.

The 2011 outbreak in China showed that poliomyelitis-free countries remain at risk for outbreaks while the poliovirus circulates anywhere in the world. Global eradication of poliomyelitis will benefit all countries, even those that are currently free of poliomyelitis.

In this work, MXene Ti3C2T x -derived nitrogen-functionalized heterophase TiO2 homojunctions (N-MXene) were prepared via the urea-involved solvothermal treatment with varying reaction time as the sensing layer to detect trace NH3 gas at room temperature (20 °C). Compared with no signal for the pristine MXene counterpart, the 18 h-treated sensors (N-MXene-18) achieved a detection limit of 200 ppb with an inspiring response that was 7.3% better than the existing MXene-involved reports thus far. Also, decent repeatability, stability, and selectivity were demonstrated. It is noteworthy that the N-MXene-18 sensors delivered a stronger response, more sufficient recovery, and quicker response/recovery speeds under a humid environment than those under dry conditions, proving the significance of humidity. Furthermore, to suppress the effect of the fluctuation of humidity on NH3 sensing during the tests, a commercial waterproof polytetrafluoroethylene (PTFE) membrane was anchored onto the sensing layer, eventually bringing about humidity-independent features. Both nitrogen doping and TiO2 homojunctions constituted by mixed anatase and rutile phases were primarily responsible for the performance improvement with respect to pristine MXene. This work showcases the enormous potential of N-MXene materials in trace NH3 detection and offers an alternative strategy to realize both heteroatom doping and partial oxidation of MXene that is applicable in future optoelectronic devices.

Investigation of Bi-doping effect on catalytic performance of Pt/3DOM-Ce1-xBixO2-δ in the aerobic oxidation of 5-hydroxymethyl-2-furfural allows us to reveal the promoted catalytically active sites: the asymmetrical oxygen vacancies coordinated with one Bi and up to three Ce cations, e.g. Bi-(-Ce)3, which can easily gain oxygen atoms in favor of the CeO2 structure, and, when filled by oxygen, easily release oxygen anions in favor of six coordination for Bi 3+ . The loss of electrons in the reduction of oxygen atoms at these sites would be replenished by electron transfer from Pt nanoparticles, eventually promoting the oxidation potential of the Pt nanoparticles. The present work points out that the promoted catalytic properties in Bi-doped CeO2 is mainly due to the asymmetric structures of the oxygen vacancies, rather than simply to the number of the oxygen vacancies. The newly proposed model of asymmetrical active sites and electron transfer mechanism may shed light on the future investigation of the physico-chemical properties of other solid solution substrate supported metal nanoparticle catalysts.

Curcumin inhibits prostate cancer by upregulating miR-143. PGK1 is downregulated by miR-143, and FOXD3 upregulation is essential for the antitumor effect of curcumin.

No abstract

Human activities and environmental deterioration have resulted in land use transition (LUT), which seriously affects the ecosystem service value (ESV) of its region. Therefore, relevant policy measures are urgently needed. Nevertheless, research on the relationships between LUTs and ESVs from the overall watershed scale is lacking. Thus, the geo-information Tupu method was applied to analyze the dynamic patterns of LUT based on land use data from 1990, 2000, 2010, and 2018 of the Yellow River Basin (YRB). Then, a newly revised ecosystem services calculation method was utilized to the responses of ESV to LUTs. The results indicated that the Tupu units of the LUT were mainly based on the mutual transformation of grassland and unused land, and cultivated land and forestland, which were widely distributed in the upper and middle reaches of the basin. The spatial distribution was concentrated, and the expansion’s trend was also obvious. Moreover, the conversion of cultivated land into construction land was mainly distributed in the lower reaches of the basin. During 1990–2018, the total ESV fluctuated and increased (+10.47 × 108 USD) in the YRB. Thereinto, the ESV of grassland (45%) and forestland (30%) made the greatest contribution to the total ESV. As for different reaches, the ESV increased in the upstream, but decreased in the midstream and the downstream. In terms of contribution rate, the conversion of unused land into grassland (12.477%) and grassland into forestland (9.856%) were the main types to enhance the ESV in the YRB, while the conversion of forestland into grassland (−8.047%) and grassland to unused land (−7.358%) were the main types to reduce the ESV. Furthermore, the range of ecological appreciation zones was widely distributed and scattered, while the range of ecological impairment zones was gradually expanded. These findings could have theoretical support and policy implications for land use planning and environmental services in the YRB.

In this study we investigated the association between intestinal dysbacteriosis with colorectal cancer progress and the underlying molecular mechanisms. Tumor progression was evaluated using xenograft mice model. The epithelial-mesenchymal transition (EMT) markers were quantified by both real-time PCR and immunoblotting. The serum content of IL-6 and TNF-α were measured with ELISA kits. Cell proliferation was determined by the Cell Counting Kit-8. Intestinal dysbacteriosis was successfully simulated by the administration of a large dose of antibiotics and was demonstrated to promote xenograft tumor growth and induce EMT. Accordingly, the serum concentrations of cytokines IL-6 and TNF-α were significantly increased. Furthermore, the production and secretion of IL-6 and TNF-α were remarkably elevated in macrophages isolated from intestinal dysbiotic mice in comparison with the normal counterparts, and conditioned medium from these was shown to significantly stimulate EMT process in HT29 cells in vitro. Macrophage depletion completely abrogated the pro-tumor effect of intestinal dysbacteriosis. Our results suggest that intestinal dysbacteriosis stimulates macrophage activation and subsequently induces EMT process via secreted pro-inflammatory cytokines IL-6 and TNF-α.