Biofilter is the core unit of the recirculating aquaculture system(RAS), which is mainly to achieve in situ purification and restoration of water pollutants by enriching microorganisms on biofilters to form biofilms. To explore the changes and metabolic characteristics of microbial communities in the process of biofilm formation in the RAS under low-temperature environment, the 16S rRNA high-throughput sequencing was applied to analyze differences of the microbial composition and structural characteristics in the biofilm initiation group (I) and biofilm formation group(B), and the PICRUSt functional gene prediction method was used to predict the metabolic functions. Results of microbial diversity showed that the OTUs numbers, Shannon index and richness index increased significantly after bio-film formation(p<0.05). The Pcoa results indicated that the microbial community composition in group B was quite different compared with the group I. After the biofilm formation, the phylum Proteobacteria, Bacteroidota, Fusobacteriota, Patescibacteria and Verrucomicrobiota were the main dominant bacteria on the biofilter. The denitrifying bacteria, such as Comamonadaceae and Rhodobacteraceae as the dominant bacteria in group B. Meanwhile, the activities of bio-film microorganisms in metabolic aspects of Carbohydrate Metabolism, Energy Metabolism, Lipid Metabolism, Amino Acid Metabolism of Terpenoids and Polyketides and Enzyme Families were obviously enhanced(p <0.05).
In order to understand the changes in the ecological environment of Miyun Reservoir after the South-to-North Water Transfer Project, our study investigated the changes in the composition and structure of two indicator species in the Miyun Reservoir and applied the rotifer biological assessment method to analyse the changes of water ecological environment. Our results showed that a total of 10 species of the genera Trichocerca and Brachionus were identified during the investigation with an average annual abundance of 542.124 ind./L and 102.68 ind./L, respectively. Among them, T. pusilla and T. cylindrica were the dominant species of the genus Trichocerca, and the majority of Brachionus species were B. diversicornis, B. angularis, B. caudatus. Variance results showed that there were significant differences in the abundance of these two rotifer pupations at different inter-annual and spatial scales during the survey. The biological evaluation indicated that the water quality has gradually changed from eutrophication to oligotrophication after the South-to-North Water Transfer Project, and the ecological environment was maintained well. Our results will provide necessary ecological parameters for water environment protection and ecological safety management of Miyun Reservoir.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.