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Computer-generated holograms written on a liquid-crystal display can be used to generate dynamic light fields of arbitrary shape. This method was used to simultaneously trap polystyrene particles laterally and to displace them independently of one another.

An optical system based on short-coherence digital holography suitable for three-dimensional (3D) microscopic investigations is described. The light source is a short-coherence laser, and the holograms are recorded on a CCD sensor. The interference (hologram) occurs only when the path lengths of the reference and the object beam are matched within the coherence length of the laser. The image of the part of the sample that matches the reference beam is reconstructed by numerical evaluation of the hologram. The advantages of the method are high numerical aperture (this means high spatial resolution), detection of the 3D shape, and a lensless imaging system. Experimental results are presented.

The phase retrieval algorithm has been used in this paper for whole reconstruction of the optical wave fields. The quantitative information of the phase distribution as well as the intensity distribution of the reconstruction field at different locations along the propagation direction has been achieved from double or multi in-line holograms. Numerical reconstructions of the wave fields from experimentally recorded in-line holograms are presented. This technique can be potentially applied for aberrated wave front analyzing and 3D imaging.

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In the image of a confocal microscope, only those parts of an object appear bright that are located in the focal plane of the objective. Because of an axial chromatic aberration deliberately introduced into the microscope objective, the location of the focal plane depends on the wavelength used. By using a white-light source and examining an object with a depth variation less than the axial range of the chromatic focus, we find that all parts of the object appear sharp and bright in the image, but according to its height they appear in different colors. A camera with black-and-white film sequentially combines; with three selected chromatic filters, intensity and tone of color of each 'Object point. For each tone of color onc can assign a height by using a calibration curve. This assignment could be made unequivocal by the selection of filters with adequate chromatic transmission.

We propose a new method for measuring large-object deformations byusing temporal evolution of the speckles in speckleinterferometry. The principle of the method is that by deformingthe object continuously, one obtains fluctuations in the intensity ofthe speckle. A large number of frames of the object motion arecollected to be analyzed later. The phase data for whole-objectdeformation are then retrieved by inverse Fourier transformation of afiltered spectrum obtained by Fourier transformation of thesignal. With this method one is capable of measuring deformationsof more than 100 mum, which is not possible using conventionalelectronic speckle pattern interferometry. We discuss theunderlying principle of the method and the results of theexperiments. Some nondestructive testing results are alsopresented.