The tumour-associated microbiota is an intrinsic component of the tumour microenvironment across human cancer types1,2. Intratumoral host–microbiota studies have so far largely relied on bulk tissue analysis1–3, which obscures the spatial distribution and localized effect of the microbiota within tumours. Here, by applying in situ spatial-profiling technologies4 and single-cell RNA sequencing5 to oral squamous cell carcinoma and colorectal cancer, we reveal spatial, cellular and molecular host–microbe interactions. We adapted 10x Visium spatial transcriptomics to determine the identity and in situ location of intratumoral microbial communities within patient tissues. Using GeoMx digital spatial profiling6, we show that bacterial communities populate microniches that are less vascularized, highly immuno‑suppressive and associated with malignant cells with lower levels of Ki-67 as compared to bacteria-negative tumour regions. We developed a single-cell RNA-sequencing method that we name INVADEseq (invasion–adhesion-directed expression sequencing) and, by applying this to patient tumours, identify cell-associated bacteria and the host cells with which they interact, as well as uncovering alterations in transcriptional pathways that are involved in inflammation, metastasis, cell dormancy and DNA repair. Through functional studies, we show that cancer cells that are infected with bacteria invade their surrounding environment as single cells and recruit myeloid cells to bacterial regions. Collectively, our data reveal that the distribution of the microbiota within a tumour is not random; instead, it is highly organized in microniches with immune and epithelial cell functions that promote cancer progression.
Heterogeneous domain adaptation (HDA) aims to exploit knowledge from a heterogeneous source domain to improve the learning performance in a target domain. Since the feature spaces of the source and target domains are different, the transferring of knowledge is extremely difficult. In this paper, we propose a novel semi-supervised algorithm for HDA by exploiting the theory of optimal transport (OT), a powerful tool originally designed for aligning two different distributions. To match the samples between heterogeneous domains, we propose to preserve the semantic consistency between heterogeneous domains by incorporating label information into the entropic Gromov-Wasserstein discrepancy, which is a metric in OT for different metric spaces, resulting in a new semi-supervised scheme. Via the new scheme, the target and transported source samples with the same label are enforced to follow similar distributions. Lastly, based on the Kullback-Leibler metric, we develop an efficient algorithm to optimize the resultant problem. Comprehensive experiments on both synthetic and real-world datasets demonstrate the effectiveness of our proposed method.
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