]adenine in the low nanomolar range. Structure-activity relationships revealed that hypoxanthine also bound with high affinity to A. xylosoxidans, whereas other nucleobases (uracil, xanthine) and nucleosides (adenosine, uridine) did not. The nature of the labeled site in bacteria is not known, but preliminary results indicate that it may be a high-affinity purine transporter. We conclude that [ 3 H]adenine is a well-suitable radioligand for adenine receptor binding studies but that bacterial contamination of the employed buffer solutions must be avoided.

The use of habitats by wild animals is commonly assumed to be decreasing due to human activities, such as tourism or the installation and use of wind-energy plants. These anthropogenic interferences may subject animals to chronic stress. To be able to objectively characterise the effects on animal populations or on individual animals, the collection of data that might be suitable to monitor such chronic stress is required. In this study of hunted red deer, we report data that are related to adrenal activity and are not affected by the acute stress induced by hunting. Adrenal glands and samples from ileal digesta were collected from 75 hunted deer from seven different habitats in the German Rhineland. The adrenal glands were evaluated histomorphometrically; in the digesta, the concentration of cortisol metabolites, i.e. of 11,17-dioxoandrostanes (11,17-DOA), was measured. Digesta were also examined for parasites. Animals were grouped according to age, sex, habitat, and hunting method. Animals were infected with gastrointestinal helminths and lungworms; examination for liver flukes was negative. Significant differences were not established among the different groups for any of the recorded parameters. For sex, a tendency (P=0.11) towards higher DOA levels was observed in female deer when compared to male deer. The variability of the parameters together with the lack of identifiable influences of hunting indicates that chronic stress might indeed have been a relevant factor. However, none of the parameters analysed can presently be validly used to evaluate habitat quality for red deer since physiological stressors cannot be differentiated.