The antimicrotubular agent docetaxel is a widely used chemotherapeutic drug for the treatment of multiple solid tumors and is predominantly dependent on hepatic disposition. In this study, we evaluated drug uptake transporters capable of transporting radiolabeled docetaxel. By screening an array of drug uptake transporters in HeLa cells using a recombinant vaccinia-based method, five organic anion transporting polypeptides (OATPs) capable of docetaxel uptake were identified: OATP1A2, OATP1B1, OATP1B3, OATP1C1, and Oatp1b2. Kinetic analysis of docetaxel transport revealed similar kinetic parameters among hepatic OATP1B/1b transporters. An assessment of polymorphisms (SNPs) in SLCO1B1 and SLCO1B3 revealed that a number of OATP1B1 and OATP1B3 variants were associated with impaired docetaxel transport. A transwell-based vectorial transport assay using MDCKII stable cells showed that docetaxel was transported significantly into the apical compartment of double transfected-(MDCKIIOATP1B1/MDR1 and MDCKII-OATP1B3/MDR1) cells compared to single transfected-(MDCKII-OATP1B1 and MDCKII-OATP1B3) cells (p<0.05) or control (MDCKII-Co) cells (p<0.001). In vivo docetaxel transport studies in Slco1b2−/− mice showed ~>5.5-fold higher plasma concentrations (p<0.01) and ~3-fold decreased liver-to-plasma ratio (p<0.05) of docetaxel compared to wild-type mice. The plasma clearance of docetaxel in Slco1b2−/− mice was 83% lower than wild-type mice (p<0.05). In conclusion, this study demonstrates the important roles of OATP1B transporters to the hepatic disposition and clearance of docetaxel, and supporting roles of these transporters for docetaxel pharmacokinetics.
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