The need for further development and deepening of research in the field of innovative activity in Ukraine is determined by hard current situation in in this direction. The purpose of the research is to justify the toolkit for evaluating the innovative activity and innovative work of the enterprise at two levels: international and national and at the micro level. The analysis of the financing of scientific research and development was conducted. It was found that, despite the existing difficulties, Ukraine has retained a strong scientific potential. It was established that innovative work in modern conditions is characterized by the appearance of new qualities that significantly change its content and expand the possibilities of its application in all functional areas of the enterprise. It is proposed to identify the features of the development of innovative activity and innovative work using the developed toolkit at two levels: first, at international and national, and second, at the micro level. The list of indicators, which are included for the assessment and analysis of the macro environment of enterprises – the global innovation index – has been researched and substantiated; indicators of the development of innovative enterprises; for the assessment and analysis of the internal microenvironment – indicators of the development of enterprises and organizations engaged in innovative work; indicators of financing innovative activity of enterprises. The obtained results of the calculations make it possible to compare the capacity of enterprises regarding innovative activity with the possibilities of the external environment, and ensure a clear establishment of goals and development priorities in the innovative sphere of the enterprise. A toolkit for evaluating the innovative activity and innovative work of the enterprise has been developed, which is characterized by the presence of the following areas of evaluation: at the international and national level and at the micro level, based on the justification of a list of indicators that allows determining both the opportunities of the external environment for the established development priorities and the ability of the enterprise to further development of the innovative sphere.