The current work investigated the ecotoxicological effects induced by Titanium Dioxide (TiO2) nanoparticles (NPs), used at three different concentrations (C1 = 10 μg·L−1, C2 = 100 μg·L−1 and C3 = 1000 μg·L−1) in a laboratory experiment, on the freshwater mussel Unio ravoisieri. Biochemical analyses of gills and digestive glands revealed a stress-related disruption of the antioxidant system. The catalase activity and the rates of malonedialdehyde and hydrogen peroxide production were significantly higher in both organs following the exposure to TiO2 NPs and was concentration-dependent. In addition, based on the observed changes in acetylcholinesterase activity, it can be concluded that the disturbance threshold for the cholinergic system was less than 1 mg·L−1 of TiO2. Overall, the results suggest that the mussel Unio ravoisieri could be used as a sentinel species in monitoring surveys assessing the environmental impact of metallic nanoparticles in freshwater systems.
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