Chrysomya bezziana this study was carried out between October, 2021 to March 31, 2022 in six region from Al-Diwaniyah province. Observe the influence of the month and type of animal on the region's. The total number of animals infected was 33 (1) cattle, (7) lambs, and (35) sheep. Al-muhnawa had the highest rate of infestation, while Al-hamza had the lowest rate of infestation. The highest rate of infestation recorded in March ()% and the lowest in February ()%.Conventional PCR (Polymerase Chain Reaction) was used for molecular identification. A DNA extraction was performed on 50 randomly chosen larvae from the total population. The size of the PCR product (548 pb) For Chrysomya bezziana, from the infected animals larvae were taken to DNA extracted and used following targeting 16S rRNA gene product size (548 bp). There follows sequence analysis to obtain the nucleotide (16S rRNA) gene, and the sequence was recoded in the National Center for Biotechnology (NCBI) with accession numbers (ON059979, ON059980, ON059981, ON059982, ON059983, ON059984, ON059985)Iraq. This gene's homology sequence identity percentage was calculated by comparing it to another globally registered gene (NCBI).
A total of 364 head sheep were examine from fourteen region in Al- Diwaniyah province 224 positive to infestation and larvae were collected and sent to the laboratory of veterinary medicine, from October 2021 to March 2022 for both sexes. The rate of Oestrus ovis infestation in sheep in the study areas was 61.53%. Al-Dighara abattoir had the highest infestation rate (82.75%) and Al-Salahia had the lowest infestation rate (17.64%). DNA extraction sequencing and phylogenetic analysis were used to identify Oestrus ovis larvae (16S rRNA) gene, the sequence was recoded in the National Center Biotechnology (NCBI) with accession numbers (ON059972, ON059973, ON059974, ON059975, ON059976, ON059977, and ON059978) Iraq for O.ovis larvae. The percentage of homology sequence identity between this gene and another global gene registered in NCBI was (99–100%) with Brazil and Bosnia and Herzegovina isolates, (97.17%) with USA isolates, and (96.17, 96.33, and 96.68%) with Brazil, Bosnia and Herzegovina, and USA isolates, respectively.
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