This study was conducted to determine the presence and distribution of Anaplasma ovis and Anaplasma phagocytophilum in small ruminants in Istanbul, Tekirdag, Edirne and Kirklareli provinces in Thrace region of northwestern Turkey during May-September in 2014. A total of 423 blood samples (216 sheep and 207 goats) were collected randomly from small ruminants regardless of the clinical symptoms. Species-specific polymerase chain reaction (PCR) assays, targeting the major surface protein 4 (msp4), were employed for identification of A. ovis and A. phagocytophilum and selected products were confirmed via sequencing. A total of 230 small ruminants (54.37%) were found to be infected with A. ovis and/or A. phagocytophilum.
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