Objective: The aim of this study was to screen for possible biomarkers of metastatic osteosarcoma (OS) using a DNA microarray. Methods: We downloaded the gene expression profile GSE49003 from Gene Expression Omnibus database, which included 6 gene chips from metastatic and 6 from non-metastatic OS patients. The R package was used to screen and identify differentially expressed genes (DEGs) between metastatic and non-metastatic OS patients. Then we compared the expression of DEGs in the two groups and sub-grouped into up-regulated and down-regulated, followed by functional enrichment analysis using the DAVID system. Subsequently, we constructed an miRNA-DEG regulatory network with the help of WebGestalt software. Results: A total of 323 DEGs, including 134 up-regulated and 189 down-regulated, were screened out. The up-regulated DEGs were enriched in 14 subcategories and most significantly in cytoskeleton organization, while the down-regulated DEGs were prevalent in 13 subcategories, especially wound healing. In addition, we identified two important miRNAs (miR-202 and miR-9) pivotal for OS metastasis, and their relevant genes, CALD1 and STX1A. Conclusions: MiR-202 and miR-9 are potential key factors affecting the metastasis of OS and CALD1 and STX1A may be possible targets beneficial for the treatment of metastatic OS. However, further experimental studies are needed to confirm our results.

This study aims to study the effect of reactions and temperature on the drag force coefficient in the process of char combustion. For this purpose, two-dimensional fully resolved simulations are performed using the ghost cell immersed boundary method.Heat and mass transfer, together with the corresponding Stefan flow, is accounted for.Reactive particles with different reaction rates, temperatures and diameters are compared with a non-reactive adiabatic particle and a particle with outflow. For a char particle, results show that reactions tend to increase the drag force, which is converse to the effect observed for non-reactive particles with a pure outflow. This discrepancy is due to the fact that species and temperature distribution play an important role, and both of them can affect the property of the fluid. Hence, a reactive particle cannot be simplified as a particle with only outflow. Based on the current study, a new drag force correlation for a single reactive particle is obtained. The correlation shows a good agreement with the simulation results. A posterior analysis is also performed to verify the accuracy of the correlation.

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Multiphase flows with momentum, heat, and mass transfer exist widely in a variety of industrial applications. With the rapid development of numerical algorithms and computer capacity, advanced numerical simulation has become a promising tool in investigating multiphase transport problems. Immersed boundary (IB) method has recently emerged as such a popular interface capturing method for efficient simulations of multiphase flows, and significant achievements have been obtained. In this review, we attempt to give an overview of recent progresses on IB method for multiphase transport phenomena. Firstly, the governing equations, the basic ideas, and different boundary conditions for the IB methods are introduced. This is followed by numerical strategies, from which the IB methods are classified into two types, namely the artificial boundary method and the authentic boundary method. Discussions on the implementation of various boundary conditions at the interphase surface with momentum, heat, and mass transfer for different IB methods are then presented, together with a summary. Then, the state-of-the-art applications of IB methods to multiphase flows, including the isothermal flows, the heat transfer flows, and the mass transfer problems are outlined, with particular emphasis on the latter two topics. Finally, the conclusions and future challenges are identified.

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