Abstract. Desipramine (DMI) has been reported to induce glucocorticoid receptor-mediated signal transduction in recent studies. It has been suggested that a non-glucocorticoid receptor signaling pathway might play an important role in skin squamous carcinoma Ca3/7 cells. The aim of this study was to investigate the growth inhibitory effects of DMI on Ca3/7 cells by evaluating the mRNA expression of genes related to apoptosis and cell cycle progression. Hoechst nuclear staining and DNA fragmentation assays were used to detect apoptosis, and the cell cycle was analyzed by flow cytometry. Apoptotic bodies in the nuclei of cells and DNA fragmentation were observed when the Ca3/7 cells were treated with 20 μM DMI for 24 h. Quantitative RT-PCR (reverse transcriptional-polymerase chain reaction) showed that DMI caused a decrease in Bcl-2 and survivin but not Bcl-xL gene expression and an increase in the expression of Bax, Apaf-1, caspase-3 and caspase-7 in a dose-and timedependent manner. DMI also caused translocation of the apoptosis-inducing factor from the cytoplasm to the nucleus as well as cell cycle arrest in the Ca3/7 cells. Quantitative RT-PCR revealed that DMI decreased the expression of the PCNA gene and caused an increase in the expression of the p21 and p27 genes in the Ca3/7 cells. Our results showed that DMI inhibited the growth of Ca3/7 cells by inducing both apoptosis and cell cycle arrest.
phosphat in einigen Tropfen Salpeters~ure, die er zun~chst auf 1 1 und dann entsprechend waiter verdt~nnt. Wasser mit hSherem Phosphors'~uregehalt muss zuvor entsprechend verdt~nnt werden, da sonst nicht bloss GelbfSrbung auftritt, sondern ein Niederschlag entsteht. Kiesels~ure muss natt~rlich vorher abgeschieden werden. Der Verfasser fOhrt einen Speeialfall an, in welchem auch ein hoher Gehalt an Phosphaten keinen Grund zur Beanstandung darbot, da derselbe aus den versteinerten Resten vorweltlicher Thiere stammte. Dutch Koehen des Wassers konnte die PhosphorsSure als Kalksalz ausgeschieden werden.
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